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JAC Advance Access published online on August 12, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh391
© 2004 by The British Society for Antimicrobial Chemotherapy
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Received June 4, 2004
Revised June 29, 2004
Accepted July 1, 2004

Brief report

Enterococcus faecium N03-0072 carries a new VanD-type vancomycin resistance determinant: characterization of the VanD5 operon

David A. Boyd 1, Pamela Kibsey 2, Diane Roscoe 3, Michael R. Mulvey 1*, on behalf of the Canadian Nosocomial Infection Surveillance Program (CNISP) {dagger}

1 Nosocomial Infections, National Microbiology Laboratory, Health Canada, 1015 Arlington Street, Winnipeg, Manitoba, R3E 3R2, Canada
2 Victoria General Hospital, Victoria, British Columbia, Canada
3 Vancouver General Hospital, Vancouver, British Columbia, Canada

* To whom correspondence should be addressed. E-mail: michael_mulvey{at}hc-sc.gc.ca.


  Abstract

Objectives: To genotypically characterize the vancomycin resistance mechanism of Enterococcus faecium N03-0072, which was negative by PCR for the currently known van genotypes.

Methods: PCR was used to amplify the entire vancomycin resistance operon and the complete nucleotide sequence was determined by dideoxy cycle sequencing.

Results: Analysis revealed a VanD-type operon with 94% nucleotide identity to the VanD4 operon and 90% nucleotide identity to the VanD1/D3 operons. A set of universal primers was designed in order to identify all current vanD variants by PCR.

Conclusions: E. faecium N03-0072 carries a new VanD-type operon, designated VanD5.

Keywords: glycopeptide resistance; van PCR; E. faecium.

{dagger}Members of the Canadian Nosocomial Infection Surveillance Program (CNISP) are listed in the Acknowledgements


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