Skip Navigation



JAC Advance Access published online on July 1, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh345
© 2004 by The British Society for Antimicrobial Chemotherapy
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
54/2/451    most recent
dkh345v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Yong, D.
Right arrow Articles by Tapsall, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yong, D.
Right arrow Articles by Tapsall, J. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received March 7, 2004
Revised May 22, 2004
Accepted June 2, 2004

Original article

Epidemiological characteristics and molecular basis of fluoroquinolone-resistant Neisseria gonorrhoeae strains isolated in Korea and nearby countries

Dongeun Yong 1, Tae Sook Kim 2, Jong Rak Choi 2, Jong Hwa Yum 3, Kyungwon Lee 4*, Yunsop Chong 5, Hee-Bok Oh 6, Tiffany Shultz 7, John W. Tapsall 7

1 Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea; Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea; Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea
2 Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea
3 Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea; Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea
4 Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea; Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea; Brain Korea 21 Project for Medical Sciences, Yonsei University College of Medicine, Seoul, Korea
5 Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea; Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea
6 Department of Bacteriology, National Institute of Health, Seoul, Korea
7 WHO Collaborating Centre for STD and HIV, Department of Microbiology, The Prince of Wales Hospital, Sydney, Australia

* To whom correspondence should be addressed. E-mail: leekcp{at}yumc.yonsei.ac.kr.


  Abstract

Objectives: This study was performed to examine the cause of the increase in quinolone-resistant Neisseria gonorrhoeae (QRNG) observed in Korea.

Methods: The antimicrobial susceptibilities of 190 isolates of gonococci from Korea in 2000 were examined by NCCLS methods, and subsets of these isolates underwent mutation analysis of the quinolone resistance-determining regions (QRDRs) of gyrA and parC. Molecular epidemiological characterization of 25 Korean isolates and 54 isolates from overseas was performed by pulsed-field gel electrophoresis (PFGE) and the results compared.

Results: Most (172, 90.5%) of the 190 gonococci tested displayed reduced susceptibility to ciprofloxacin. All strains with high-level ciprofloxacin resistance (ciprofloxacin MIC ≥ 4 mg/L) contained a double amino acid alteration at the 91 and 95 positions in the QRDR of GyrA and a single alteration in ParC. PFGE types of high-level QRNG in Korea were mostly different from those of other nearby countries.

Conclusions: These results suggest that the observed increase in ciprofloxacin-resistant isolates is due to the mutation and spread of Korean multiclonal isolates rather than importation from overseas.

Keywords: N. gonorrhoeae; pulsed-field gel electrophoresis; ciprofloxacin; resistance; QRDRs.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
D. M. Whiley, E. A. Limnios, S. Ray, T. P. Sloots, and J. W. Tapsall
Diversity of penA Alterations and Subtypes in Neisseria gonorrhoeae Strains from Sydney, Australia, That Are Less Susceptible to Ceftriaxone
Antimicrob. Agents Chemother., September 1, 2007; 51(9): 3111 - 3116.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
B. Wang, J.-s. Xu, C.-x. Wang, Z.-h. Mi, Y.-p. Pu, M. Hui, T. K. W. Ling, and C.-Y. Chan
Antimicrobial susceptibility of Neisseria gonorrhoeae isolated in Jiangsu Province, China, with a focus on fluoroquinolone resistance.
J. Med. Microbiol., September 1, 2006; 55(Pt 9): 1251 - 1255.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. Takei, Y. Yamaguchi, H. Fukuda, M. Yasuda, and T. Deguchi
Cultivation of Neisseria gonorrhoeae in Liquid Media and Determination of Its In Vitro Susceptibilities to Quinolones
J. Clin. Microbiol., September 1, 2005; 43(9): 4321 - 4327.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
K. A. Talukder, B. K. Khajanchi, M. A. Islam, D. K. Dutta, Z. Islam, A. Safa, G. Y. Khan, K. Alam, M. A. Hossain, S. Malla, et al.
Genetic relatedness of ciprofloxacin-resistant Shigella dysenteriae type 1 strains isolated in south Asia
J. Antimicrob. Chemother., October 1, 2004; 54(4): 730 - 734.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.