Skip Navigation



JAC Advance Access published online on July 8, 2004

Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh333
© 2004 by The British Society for Antimicrobial Chemotherapy
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
54/2/429    most recent
dkh333v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Antunes, P.
Right arrow Articles by Peixe, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Antunes, P.
Right arrow Articles by Peixe, L.
Social Bookmarking
 Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received February 3, 2004
Revised May 14, 2004
Accepted May 19, 2004

Original article

Dissemination amongst humans and food products of animal origin of a Salmonella typhimurium clone expressing an integron-borne OXA-30 {beta}-lactamase

Patrícia Antunes 1, Jorge Machado 2, João Carlos Sousa 3, Luísa Peixe 3*

1 Faculdade de Ciências da Nutrição e Alimentação, Universidade do Porto, Porto, Portugal; Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, no. 164, 4050-047 Porto, Portugal
2 Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal
3 Laboratório de Microbiologia, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, no. 164, 4050-047 Porto, Portugal

* To whom correspondence should be addressed. E-mail: lpeixe{at}ff.up.pt.


   Abstract

Objectives: Characterization of the molecular basis for {beta}-lactam resistance and evaluation of the clonal relatedness among nine isolates of multidrug-resistant Salmonella typhimurium recovered from seven clinical human samples and two pork end products.

Methods: The isolates were examined for susceptibility to antimicrobial agents. The relationships between resistance genes, class 1 integrons, plasmids and isolates were screened by molecular methods such as polymerase chain reaction and restriction fragment length analysis.

Results: A blaOXA-30 gene, located in a class 1 integron, was detected in all isolates. This integron was present on a conjugative plasmid in all but one isolate. By pulsed-field gel electrophoresis, it was determined that all strains share the same chromosomal type.

Conclusions: This study demonstrates the spread of an OXA-30-producing S. typhimurium in Portugal, suggesting dissemination of a resistant clone through the food chain.

Keywords: salmonellosis; food-borne diseases; class 1 integrons; antimicrobial resistance; {beta}-lactams.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
Antimicrob. Agents Chemother.Home page
A. Herrero, M. C. Mendoza, R. Rodicio, and M. R. Rodicio
Characterization of pUO-StVR2, a Virulence-Resistance Plasmid Evolved from the pSLT Virulence Plasmid of Salmonella enterica Serovar Typhimurium
Antimicrob. Agents Chemother., December 1, 2008; 52(12): 4514 - 4517.
[Abstract] [Full Text] [PDF]


Home page
Antimicrob. Agents Chemother.Home page
E. Machado, J. Ferreira, A. Novais, L. Peixe, R. Canton, F. Baquero, and T. M. Coque
Preservation of Integron Types among Enterobacteriaceae Producing Extended-Spectrum {beta}-Lactamases in a Spanish Hospital over a 15-Year Period (1988 to 2003)
Antimicrob. Agents Chemother., June 1, 2007; 51(6): 2201 - 2204.
[Abstract] [Full Text] [PDF]


Home page
J Antimicrob ChemotherHome page
J.-C. Pan, R. Ye, D.-M. Meng, W. Zhang, H.-Q. Wang, and K.-Z. Liu
Molecular characteristics of class 1 and class 2 integrons and their relationships to antibiotic resistance in clinical isolates of Shigella sonnei and Shigella flexneri
J. Antimicrob. Chemother., August 1, 2006; 58(2): 288 - 296.
[Abstract] [Full Text] [PDF]


Home page
J Antimicrob ChemotherHome page
G. Peirano, Y. Agerso, F. M. Aarestrup, E. M. F. dos Reis, and D. dos Prazeres Rodrigues
Occurrence of integrons and antimicrobial resistance genes among Salmonella enterica from Brazil
J. Antimicrob. Chemother., August 1, 2006; 58(2): 305 - 309.
[Abstract] [Full Text] [PDF]


Home page
J Antimicrob ChemotherHome page
P. Antunes, J. Machado, and L. Peixe
Characterization of antimicrobial resistance and class 1 and 2 integrons in Salmonella enterica isolates from different sources in Portugal
J. Antimicrob. Chemother., August 1, 2006; 58(2): 297 - 304.
[Abstract] [Full Text] [PDF]


Home page
J. Clin. Microbiol.Home page
F.-X. Weill, F. Guesnier, V. Guibert, M. Timinouni, M. Demartin, L. Polomack, and P. A. D. Grimont
Multidrug Resistance in Salmonella enterica Serotype Typhimurium from Humans in France (1993 to 2003).
J. Clin. Microbiol., March 1, 2006; 44(3): 700 - 708.
[Abstract] [Full Text] [PDF]


Home page
J Antimicrob ChemotherHome page
A. Herrero, M. R. Rodicio, M. A. Gonzalez-Hevia, and M. C. Mendoza
Molecular epidemiology of emergent multidrug-resistant Salmonella enterica serotype Typhimurium strains carrying the virulence resistance plasmid pUO-StVR2
J. Antimicrob. Chemother., January 1, 2006; 57(1): 39 - 45.
[Abstract] [Full Text] [PDF]


Home page
Annals of Clinical & Laboratory ScienceHome page
Y.-R. Kim, S.-I. Kim, J.-Y. Lee, Y.-J. Park, K.-Y. Lee, and M.-W. Kang
NosocomialTransmission of CTX-M-15 and OXA-30 {beta}-Lactamase-Producing Escherichia coli in a Neurosurgical Intensive Care Unit
Ann. Clin. Lab. Sci., January 1, 2005; 35(3): 297 - 301.
[Abstract] [Full Text] [PDF]



Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.