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JAC Advance Access published online on June 9, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh320
© 2004 by The British Society for Antimicrobial Chemotherapy
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Received February 17, 2004
Revised May 4, 2004
Accepted May 10, 2004

Original article

Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis

Dihadenys Lemus 1, Anandi Martin 2*, Ernesto Montoro 1, Françoise Portaels 2, Juan Carlos Palomino 2

1 Instituto de Medicina Tropical ‘Pedro Kouri’, La Habana, Cuba
2 Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat, 155, Antwerp, B-2000 Belgium

* To whom correspondence should be addressed. E-mail: amartin{at}itg.be.


  Abstract

Objective: To study the performance of three rapid low cost methods for the detection of rifampicin resistance.

Methods: A panel of 20 coded Mycobacterium tuberculosis strains was tested blindly by the low cost methods: nitrate reductase, MTT and resazurin assays, and compared with the results obtained with the gold standard methods: the proportion method on Löwenstein-Jensen medium and the BACTEC TB 460 system. We have also tested two commercial tests: MGIT and INNO LiPA Rif.TB kit.

Results: Complete agreement was observed among all methods.

Conclusion: These three simple methods might become inexpensive alternative procedures for rapid detection of rifampicin resistance in low-resource countries.

Key Words: M. tuberculosis, MTT, resazurin, nitrate reductase assay, rifampicin


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