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JAC Advance Access published online on June 9, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh291
© 2004 by The British Society for Antimicrobial Chemotherapy
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Received January 2, 2004
Revised March 26, 2004
Accepted April 14, 2004

Brief report

Application of microdialysis to cancellous bone tissue for measurement of gentamicin levels

Lars B. Stolle 1*, Magnus Arpi 2, Peter Holmberg-Jørgensen 3, Per Riegels-Nielsen 4, Johnny Keller 3

1 Institute of Experimental Clinical Research, Aarhus University Hospital, Skejby Hospital, Brendstrupgaardvej, 8200 Aarhus, Denmark
2 Department of Clinical Microbiology, Aarhus University Hospital, Skejby Hospital, Brendstrupgaardvej, 8200 Aarhus, Denmark
3 Department of Orthopaedic Surgery, Aarhus University Hospital, Aarhus Hospitals, Nørrebrogade 44, 8000 Aarhus C, Denmark
4 Department of Orthopaedic Surgery, Esbjerg/Varde Hospital, Østergade 8, 6700 Esbjerg, Denmark

* To whom correspondence should be addressed. E-mail: stolle{at}iekf.au.dk.


  Abstract

Objectives: Knowledge concerning the distribution of antibiotics in bone tissue is valuable for pharmacokinetic and clinical use. Unfortunately, appropriate techniques are difficult to apply. We introduced microdialysis catheters to cancellous bone tissue for the investigation of gentamicin levels and compared the pharmacokinetics measured with values obtained from bone samples.

Methods: After two microdialysis catheters had been inserted into cancellous bone, eight pigs received an intravenous bolus of 240 mg of gentamicin. Microdialysates and bone samples were obtained over a period of 6 h and drug concentrations were measured.

Results: The area under the curves of the two microdialysates and bone samples were 1569, 1721 and 1533 mg·min/L (ANOVA, P=0.81). Reproducibility of the measurements from the microdialysates was defined as the mean ratio of AUC6/catheter no. 1/AUC6/catheter no. 2. This ratio was 1.02.

Conclusions: Microdialysis is a suitable, relatively non-invasive and reproducible technique for dynamic and quantitative measurement of gentamicin levels in experimental research.

Key Words: Keywords: experimental research, bioassays, bone samples, pharmacokinetics


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