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JAC Advance Access published online on April 29, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh215
© 2004 by The British Society for Antimicrobial Chemotherapy
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Received November 21, 2003
Accepted February 27, 2004

Original article

Antimicrobial activity of clofazimine is not dependent on mycobacterial C-type phospholipases

M. C. Bopape 1, H. C. Steel 2, R. Cockeran 2, N. M. Matlola 2, P. B. Fourie 1, R. Anderson 2*

1 Tuberculosis Research Lead Programme, Medical Research Council, Pretoria;
2 Medical Research Council Unit for Inflammation and Immunity, Department of Immunology, Faculty of Health Sciences, University of Pretoria, PO Box 2034, Pretoria 0001, South Africa

* To whom correspondence should be addressed. E-mail: randerso{at}medic.up.ac.za.


  Abstract

We have used a phospholipase C (PLC)-deletion mutant (plcABC) of the H37Rv strain of Mycobacterium tuberculosis (MTB), as well as a plcA-insertion mutant of Mycobacterium smegmatis, to investigate the possible involvement of PLCs in clofazimine-mediated inhibition of mycobacterial K+ transport and growth. Inactivation of the PLCs of MTB and insertion of the plcA gene into M. smegmatis resulted in a substantial reduction and increase in hydrolysis of phosphatidylcholine (PC), respectively. However, both the mutant and wild-type strains of MTB and M. smegmatis were equally sensitive to the inhibitory effects of clofazimine on K+ uptake and growth. These observations demonstrate that the PLCs of MTB are not involved in the antimicrobial activity of clofazimine.

Key Words: Keywords: mycobacteria, phospholipase C, Mycobacterium tuberculosis, Mycobacterium smegmatis


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