JAC Advance Access published online on March 31, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh197
© 2004 by The British Society for Antimicrobial Chemotherapy
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Original article
1 Department of Microbiology,
School of Medicine, University of Patras, Rion 26500, Patras;
* Corresponding author. E-mail: spiliopl{at}med.upatras.gr.
Received 17 September 2003
; revised 14 January 2004
; accepted 24 February 2004
Objectives: Macrolide, lincosamide
and streptogramin type B (MLSB) resistance was determined
in Staphylococcus aureus clinical isolates from
two University Hospitals. Methods: Antibiotic resistance was investigated
by double disc diffusion and MIC determination. Resistance determinants
were detected by PCR and DNA hybridization, while clonal types were
identified by pulsed-field gel electrophoresis (PFGE) analysis of SmaI DNA fragments. Results: Among methicillin-susceptible S.
aureus (MSSA) isolates, inducible and MS phenotypes were detected,
with the predominance of the erm(A) gene, followed
by the msr(A) and erm(C) genes.
The majority of methicillin-resistant S. aureus (MRSA)
isolates expressed the constitutive phenotype and carried the erm(C) gene. PFGE revealed the dissemination of
two major clones among the MRSA in both hospitals. Conclusions: erm(C) is the
predominant genetic determinant for the expression of MLSB resistance
among S. aureus isolates, especially MRSA, in Greece.
This is due to the spread of two major clones in the country.
Keywords: erythromycin, clindamycin, staphylococci, mechanisms,
typing
erm(C) is the predominant genetic
determinant for the expression of resistance to macrolides among
methicillin-resistant Staphylococcus aureus clinical
isolates in Greece
2 Department of Microbiology, School
of Medicine, University of Thessalia, Larissa, Greece
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