JAC Advance Access published online on May 5, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh193
© 2004 by The British Society for Antimicrobial Chemotherapy
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1 Molecular Diagnostics
Unit, Cork Institute of Technology, Bishopstown, Cork;
* To whom correspondence should be addressed. E-mail: sfanning{at}ucd.ie.
Objectives: In this study a large
random collection (n = 378) of Irish thermophilic Campylobacter isolates were investigated for the
presence of integrons, genetic elements associated with the dissemination
of antimicrobial resistance. Methods: Purified genomic DNA from each isolate
was analysed by PCR for the presence of class 1 integrons. Four
gene cassette-associated amplicons were completely characterized. Results: Sixty-two of the isolates possessed
a complete class 1 integron with a recombined gene cassette located
within a 1.0 kb amplicon containing an aadA2 gene.
This cassette was present in both Campylobacter jejuni and Campylobacter coli isolates and following sequence
analysis was shown to be similar to sequences recently reported
in Salmonella enterica Hadar and on an 85 kb plasmid
conferring quinolone resistance in Escherichia coli. Conclusions: Aminoglycoside aadA2-encoding
class 1 integrons were identified among unrelated Campylobacter spp.
Amino acid sequence comparisons revealed identical structures in
both Salmonella and E.
coli. The presence of class 1 integrons in Campylobacter spp.
may be significant should these organisms enter the food chain and
especially when antimicrobial treatment for severe infections is
being considered.
Revised February 18, 2004
Accepted February 23, 2004
Original article
Molecular characterization of class 1 integrons
from Irish thermophilic Campylobacter spp
2 Molecular Diagnostics
Unit, Cork Institute of Technology, Bishopstown, Cork; Department of Medical Microbiology,
Cork University Hospital, Wilton, Cork;
3 Department of Medical Microbiology,
Cork University Hospital, Wilton, Cork;
4 Irish Equine Centre, Naas, Co. Kildare;
5 Molecular Diagnostics
Unit, Cork Institute of Technology, Bishopstown, Cork; Centre for Food Safety, Faculties
of Agriculture, Medicine and Veterinary Medicine, University College,
Belfield, Dublin 4, Ireland
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