JAC Advance Access published online on April 8, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh185
© 2004 by The British Society for Antimicrobial Chemotherapy
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Original article
1 Laboratoire de Bactériologie-Virologie, Centre Hospitalier Universitaire,
35033 Rennes cedex 9, France
* Corresponding author. E-mail: pierre-yves.donnio{at}chu-rennes.fr.
Received 1 August 2003
; revised 6 February 2004
; accepted 13 February 2004
Background: In this study, we investigated
the relationship between changes in antibiotic resistance and distribution
of staphylococcal cassette chromosome (SCC) types amongst methicillin-resistant Staphylococcus aureus (MRSA) isolates expressing
the most frequently encountered profiles of antibiotic resistance over
an 11 year period in the University Hospital of Rennes, France. Methods: Antibiotic susceptibilities were determined
by agar diffusion. SCC typing was performed using PCR. PFGE demonstrated
that isolates were phylogenetically related. Results: Fourteen profiles of antibiotic resistance
were defined among MRSA isolates. For each resistance profile, only
one SCC type was associated: four patterns corresponded to SCC type
I or IA, nine to SCC type IV or IVA, and none to types II and III.
One was not typed. PFGE indicated that isolates with SCC type I
or IA belong to a single genetic lineage, which also includes most
of the epidemic isolates, which carry SCC type IVA. In contrast
to type I or IA, isolates with SCC type IV or IVA were found to
be associated with several different PFGE clusters, although not
all of these represent epidemic isolates. Conclusions: During the course of the study,
the spectrum of antibiotic resistance in MRSA isolates decreased.
This occurred due to the emergence of strains with SCC type IV or
IVA, which are susceptible to more antibiotics than type I or IA
strains. The greater prevalence of such isolates could not be linked
conclusively to the presence of SCC type IV or IVA, or to one particular
PFGE cluster.
Keywords: S. aureus, MRSA, mecA,
SCC, resistance profiles
Changes in staphylococcal cassette chromosome type
and antibiotic resistance profile in methicillin-resistant Staphylococcus
aureus isolates from a French hospital over an 11 year period
2 Laboratoire de Parasitologie,
Centre Hospitalier Universitaire,
35033 Rennes cedex 9, France
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