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JAC Advance Access published online on March 31, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh172
© 2004 by The British Society for Antimicrobial Chemotherapy
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© 2004 The British Society for Antimicrobial Chemotherapy

Original article

A comparative study of the disc diffusion method with the broth microdilution and Etest methods for voriconazole susceptibility testing of Aspergillus spp

M. C. Serrano 1 , M. Ramírez 1 , D. Morilla 1 , A. Valverde 1 , M. Chávez 1 , A. Espinel-Ingroff 2 , R. Claro 3 , A. Fernández 3 , C. Almeida 3 , and E. Martín-Mazuelos 1 *

1 Servicio de Microbiología, Hospital Universitario de Valme, Ctra Cádiz s/n, Sevilla 41014;
2 Division of Infectious Diseases, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 2329-80049, USA
3 Servicio de Bioestadística, Unidad Investigación, Hospital Universitario de Valme, Sevilla, Spain;

* Corresponding author. E-mail: estrella.martin.sspa{at}juntadeandalucia.es.

Received 3 November 2003 ; revised 28 January 2004 ; accepted 8 February 2004

Abstract

Objective: The activity of voriconazole against Aspergillus spp. (n = 77) was tested by the Etest, disc diffusion and the NCCLS M38-A methods.

Methods: Four Rhizomucor spp. isolates were included to study the suitability of the three susceptibility testing methods to detect isolates resistant to voriconazole. The disc diffusion method performed on Mueller-Hinton agar (Difco) supplemented with 2% glucose and Methylene Blue (0.5 mg/L) gave zone diameters with very clear border edges.

Results: The disc diffusion method showed excellent correlation with the Etest and the NCCLS methods.

Conclusion: On the basis of our results, it appears that the disc diffusion test is a useful method for testing the activity of voriconazole against Aspergillus spp.

Keywords: antifungals, susceptibility tests
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