JAC Advance Access published online on February 18, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh134
© 2004 by The British Society for Antimicrobial Chemotherapy
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Brief report
1 Päijät-Häme
Central Hospital, Department of Clinical Microbiology, Keskussairaalankatu
7, FIN-15850 Lahti;
* Corresponding author. E-mail: pauliina.karpanoja{at}phks.fi.
Received 16 September 2003
; revised 7 January 2004
; accepted 8 January 2004
Objectives: The objective of this
multicentre study was to define the accuracy and reproducibility
of the NCCLS disc diffusion method for Haemophilus influenzae against
ampicillin and co-amoxiclav in Finnish clinical microbiology laboratories.
Special attention was paid to the ability of the laboratories to
detect Methods: Three BLNAR and two Results: The low-strength discs for ampicillin
and co-amoxiclav categorized more accurately BLNAR and BLNAS H.
influenzae isolates than did the high-strength discs recommended
by the NCCLS. In addition, the high-strength discs produced more
major errors than the low-strength discs (22 versus six for ampicillin
and 40 versus seven for co-amoxiclav). Great variation occurred
in the method regardless of the antibiotic concentration of the
discs. Conclusions: The use of low-content ampicillin
and co-amoxiclav discs is recommended for the susceptibility testing
of H. influenzae. Interpretative criteria of S
Keywords: H. influenzae, BLNAR, susceptibility
testing
Disc diffusion susceptibility testing of Haemophilus
influenzae
by NCCLS methodology using low-strength ampicillin and
co-amoxiclav discs
2 Keski-Suomi
Central Hospital, Jyväskylä;
3 National Public Health Institute,
Turku, Finland
-lactamase-negative
ampicillin-resistant (BLNAR) strains.-lactamase-negative
ampicillin-susceptible isolates (BLNAS)--originating from
the American Type Culture Collection (ATCC) and UK National External
Quality Assessment (UKNEQAS) schemes--were included in this
study. Susceptibility tests for these isolates were performed in
26 clinical microbiology laboratories, in accordance with NCCLS
guidelines. Additionally, low-strength discs for ampicillin (2 µg) and co-amoxiclav (3 µg)
were tested.
17 mm and R
13
mm for both discs are suggested.
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