JAC Advance Access published online on February 18, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh120
© 2004 by The British Society for Antimicrobial Chemotherapy
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Original article
1 Section of Microbiology
and Infectious Diseases, Kaohsiung Veterans General Hospital, 386
Ta-Chung 1st Rd, Kaohsiung; Department
of Medical Technology, Foo-Yin Institute of Technology, Kaohsiung;
Received 10 September 2003
; revised 15 December 2003
; accepted 18 December 2003
Objectives: Tentative standards for
testing MICs for Mycobacterium tuberculosis include
agar dilution and the BACTEC method. However, the conventional agar
dilution method requires 3-5 weeks to complete; whereas
BACTEC, although a rapid test, involves the use of radioisotopes.
In contrast, the MGIT 960 system uses a fluorescence quenching based
oxygen sensor that can be read automatically. This system is not
only robust, safe and simple, but has been validated for susceptibility
tests of first-line antituberculous agents. Methods: We evaluated 46 clinical strains of M. tuberculosis isolated from patients admitted
to Kaohsiung Veterans General Hospital. Testing of MICs of ciprofloxacin
and ethionamide was carried out by MGIT 960 and compared with the
agar dilution method. Results: Good agreement was found between MGIT
960 and agar dilution. The greatest concordance between the agar
dilution and MGIT assay at ±1 and ±2 dilution was 80.4% and 97.8% for
ciprofloxacin, and 82.6% and 93.5% for ethionamide,
respectively. Conclusion: MGIT 960 was found to be comparable
to the current NCCLS standard method, agar dilution, and has the
advantage of being rapid (obtaining results within 5-17
days, average 8.9 days) and easy to achieve standardization.
Keywords: minimum inhibitory concentration, M.
tuberculosis, susceptibility testing
Use of MGIT 960 for rapid quantitative measurement
of the susceptibility of Mycobacterium tuberculosis complex
to ciprofloxacin and ethionamide
2 Section of Microbiology
and Infectious Diseases, Kaohsiung Veterans General Hospital, 386
Ta-Chung 1st Rd, Kaohsiung;
3 Section of Microbiology
and Infectious Diseases, Kaohsiung Veterans General Hospital, 386
Ta-Chung 1st Rd, Kaohsiung; Graduate Institute of Environmental
Education, National Kaohsiung Normal University;
4 Section of Microbiology
and Infectious Diseases, Kaohsiung Veterans General Hospital, 386
Ta-Chung 1st Rd, Kaohsiung; Department of Medicine, National
Yang Ming University, Taipei, Taiwan, ROC
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