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JAC Advance Access published online on January 16, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh090
© 2004 by The British Society for Antimicrobial Chemotherapy
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© 2004 The British Society for Antimicrobial Chemotherapy

Original article

Biofilms, homoserine lactones and biocide susceptibility

Harriet G. MacLehose 1 , Peter Gilbert 1 , and David G. Allison 1 *

1 School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Oxford Road, Manchester M13 9PL, UK

* Corresponding author. E-mail: david.allison{at}man.ac.uk.

Received 23 July 2003 ; revised 27 November 2003 ; accepted 28 November 2003

Abstract

Aims: To investigate the susceptibility, to a range of different biocides, of Pseudomonas aeruginosa strains variously deficient in N-acyl homoserine lactone systems, grown either as planktonic or biofilm populations.

Methods and results: Biocide susceptibility data were generated for strains of P. aeruginosa deficient in N-acyl homoserine lactone production, grown planktonically or as biofilm populations using a poloxamer hydrogel construct. Component cells from the biofilm constructs were also tested for their susceptibility. Significant differences in susceptibility were noted between the wild-type strain, a mutant defective in the long chain (C-12) homoserine lactone and a mutant defective in the short chain (C-4) homoserine lactone which could not be related to the biofilm mode of growth. Moreover, differences in susceptibility appeared to be dependent upon the nature of the homoserine lactone deletion and type of biocide rather than the mode of growth.

Conclusions: No general trend exists between homoserine lactone deficiency and biocide susceptibility regardless of mode of growth.

Keywords: biocide susceptibility, N-acyl homoserine lactones, bronopol, chlorhexidine diacetate, cetrimide USP, isothiazolones
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