Skip Navigation



JAC Advance Access published online on January 16, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh077
© 2004 by The British Society for Antimicrobial Chemotherapy
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
53/2/197    most recent
dkh077v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Avison, M. B.
Right arrow Articles by Bennett, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Avison, M. B.
Right arrow Articles by Bennett, P. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2004 The British Society for Antimicrobial Chemotherapy

Original article

Role of the ‘cre/blr-tag’ DNA sequence in regulation of gene expression by the Aeromonas hydrophila {beta}-lactamase regulator, BlrA

Matthew B. Avison 1 *, Pannika Niumsup 1 , Kurshid Nurmahomed 1 , Timothy R. Walsh 1 , and Peter M. Bennett 1

1 Bristol Centre for Antimicrobial Research and Evaluation, Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, University Walk, Bristol BS8 1TD, UK

* Corresponding author. E-mail: Matthewb.Avison{at}bris.ac.uk.

Received 5 August 2003 ; revised 7 November 2003 ; accepted 17 November 2003

Abstract

Objectives: To further understand the mechanisms used to regulate expression of the blr regulon of Aeromonas hydrophila T429125, including three unlinked {beta}-lactamase genes, ampH, cepH and imiH, and to examine the role of the ‘cre/blr-tag’ DNA sequence (TTCAC) in transcriptional control exerted by the two-component system, BlrAB.

Methods: Genes linked to blrAB-ampH were cloned using standard methods; gene expression was measured by RT-PCR or {beta}-lactamase assays; transcription start sites were determined by reversed-transcript analysis; cepH promoter probe reporter constructs including cre/blr-tag deletions were generated by PCR; and BlrA was overexpressed in Escherichia coli using the pBAD plasmid.

Results: The blrD gene, encoding a putative inner membrane protein, was found to be located downstream of blrAB-ampH. RT-PCR analysis showed that blrD is part of the A. hydrophila blr regulon, and transcript start-point determinations revealed that blr-regulon promoters (including that of blrD) are preceded by at least one cre/blr-tag. Targeted deletion of the 16 bp cepH cre/blr-tag dimer blocked BlrA-induced overproduction of cepH in E. coli.

Conclusions: This is the first report of non-{beta}-lactamase genes being co-ordinately regulated with a normally co-resident {beta}-lactamase gene, and the first direct evidence for a role of the cre/blr-tag sequence in the regulation of transcription by BlrA.

Keywords: blr regulon, cre regulon, CreBC, BlrD, cre/blr-tag
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
P. D. Lister, D. J. Wolter, and N. D. Hanson
Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms
Clin. Microbiol. Rev., October 1, 2009; 22(4): 582 - 610.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
G. A. Jacoby
AmpC {beta}-Lactamases
Clin. Microbiol. Rev., January 1, 2009; 22(1): 161 - 182.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. J. L. Cariss, A. E. Tayler, and M. B. Avison
Defining the Growth Conditions and Promoter-Proximal DNA Sequences Required for Activation of Gene Expression by CreBC in Escherichia coli
J. Bacteriol., June 1, 2008; 190(11): 3930 - 3939.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
D. Girlich, T. Naas, and P. Nordmann
Regulation of class D beta-lactamase gene expression in Ralstonia pickettii.
Microbiology, September 1, 2006; 152(Pt 9): 2661 - 2672.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.