JAC Advance Access published online on January 16, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh073
© 2004 by The British Society for Antimicrobial Chemotherapy
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Brief report
1 Division of Clinical Research,
National Health Research Institutes;
* Corresponding author. E-mail: lksiu{at}mail.nhri.org.tw.
Received 30 October 2003
; revised 17 November 2003
; accepted 17 November 2003
Objective: To determine the mechanism
of rifampicin resistance in Streptococcus pneumoniae in
Taiwan. Methods: Rifampicin resistance was investigated
with respect to the rpoB gene in 23 invasive S. pneumoniae isolates collected from 1996 to 2001.
PCR and molecular typing were used for genetic and epidemiological analyses.
Transformation was used to determine the functional gene for resistance. Results: Twenty-two of 23 isolates carried at
least one mutation at either cluster I or III of rpoB;
the most frequent mutation found in 21 isolates (91%) was
histidine (H499) to asparagine or tyrosine at position 499, followed
by isoleucine to valine (I624V) at position 624 in 16 isolates (70%),
tyrosine to phenylalanine (Y589F) at position 589 in 14 isolates
(60.9%) and isoleucine to valine (I608V) at position 608
in 13 isolates (56.5%). Less-frequent mutations were also
identified: D489V, R597F, N623E, N623S, N669D, Q671K, Y674F and A683V.
High-level rifampicin resistance was observed in isolates with a
mutation at position 499 or 489. Mutations other than at position
499 or 489 played little role in or had no relation to rifampicin
resistance. No dominant epidemic strain was observed with ribotyping,
multilocus sequence typing, or serotyping. Conclusions: Rifampicin resistance among multidrug-resistant S. pneumoniae in Taiwan was mostly caused by rpoB mutations.
Keywords: S. pneumoniae, rifampicin resistance, rpoB gene
Mutations of the rpoB gene in
rifampicin-resistant
Streptococcus pneumoniae in Taiwan
2 Section of Infectious Diseases, Department of Medicine,
Taipei Veterans General Hospital and National Yang-Ming University;
3 Division of Infectious Diseases and
Tropical Medicine, Department of Internal Medicine, Tri-Services
General Hospital, Taipei, Taiwan
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Mullin, D. M. Rothstein, and C. K. Murphy Activity of Novel Benzoxazinorifamycins against Rifamycin-Resistant Streptococcus pyogenes. Antimicrob. Agents Chemother., May 1, 2006; 50(5): 1908 - 1909. [Full Text] [PDF]
|
|
|
|
|
|
M. J. Ferrandiz, C. Ardanuy, J. Linares, J. M. Garcia-Arenzana, E. Cercenado, A. Fleites, A. G. de la Campa, and the Spanish Pneumococcal Infection Study Network New Mutations and Horizontal Transfer of rpoB among Rifampin-Resistant Streptococcus pneumoniae from Four Spanish Hospitals Antimicrob. Agents Chemother., June 1, 2005; 49(6): 2237 - 2245. [Abstract] [Full Text] [PDF]
|
|