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JAC Advance Access published online on January 16, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh066
© 2004 by The British Society for Antimicrobial Chemotherapy
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© 2004 The British Society for Antimicrobial Chemotherapy

Brief report

A comparative study of the post-antifungal effect (PAFE) of amphotericin B, triazoles and echinocandins on Aspergillus fumigatus and Candida albicans

Elias K. Manavathu 1 *, Mayur S. Ramesh 1 , Inthumathi Baskaran 1 , Latha T. Ganesan 1 , and Pranatharthi H. Chandrasekar 1

1 Division of Infectious Diseases, Department of Internal Medicine, 427 Lande Building, Wayne State University, 550 E. Canfield, Detroit, MI 48201, USA

* Corresponding author. E-mail: aa1388{at}wayne.edu.

Received 29 April 2003 ; revised 15 October 2003 ; accepted 12 November 2003

Abstract

Objectives: To study the post-antifungal effect (PAFE) of antifungal drugs on Aspergillus fumigatus by a radiometric assay and compare the results with those obtained for Candida albicans.

Methods: A. fumigatus cultures pregrown for 48 h in 96-well microtitre plate were exposed to various concentrations of the antifungal drug for 2 h. The drug-treated mycelia were washed, incubated in RPMI 1640 containing 14C-labelled amino acids and the accumulation of radioactivity in the mycelia at different time intervals was determined. The PAFE was determined by plotting the amount of radioactivity associated with the mycelia against post-treatment incubation time. The PAFE of antifungal drug on C. albicans was examined by determining the multiplication (cfu/mL) of drug-pretreated cells at different time intervals for 24 h in drug-free medium.

Results: Amphotericin B produced a prolonged PAFE (7.5 ± 0.70 h) against A. fumigatus whereas itraconazole (0.5 ± 0.0 h), voriconazole (0.5 ± 0.0 h), posaconazole (0.75 ± 0.35 h), ravuconazole (0.38 ± 0.17 h) and the echinocandins caspofungin (<=0.5 h) and micafungin (<=0.5 h) produced short PAFE. Short exposure (1 h) of C. albicans to low concentrations (0.125-1 mg/L) of amphotericin B (5.3 ± 1.15 h), caspofungin (5.6 ± 0.57 h) and micafungin (5 ± 1.0 h) produced prolonged PAFE whereas the triazoles produced a short (<=0.5 h) PAFE.

Conclusions: Determination of 14C-labelled amino acid accumulation in antifungal drug-pretreated mycelia is a suitable method for studying PAFE in A. fumigatus. Antifungal drugs with fungicidal activity tend to possess longer PAFE compared to fungistatic drugs.

Keywords: caspofungin, micafungin, azole antifungals, susceptibility testing, antifungal drugs
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