JAC Advance Access published online on January 16, 2004
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh063
© 2004 by The British Society for Antimicrobial Chemotherapy
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Original article
1 Department of Biological
Sciences, Box 70703, East Tennessee State University, Johnson City, TN 37614
* Corresponding author. E-mail: levyf{at}etsu.edu.
Received 18 July 2003
; revised 7 November 2003
; accepted 10 November 2003
Objectives: The hypothesis that BRO-1
selectively replaced the BRO-2 isoform of the Moraxella
catarrhalis BRO Methods: A rapid, one-step PCR assay conducted
on 354 isolates spanning 1984-1994 distinguished bro alleles
in over 97% of the Results: BRO-2 isolates comprised 0-10% of
the population per year with no evidence of a decline over time. All Conclusions: The relative rarity of BRO-2 throughout
the study, the absence of a declining temporal trend, and genetic
diversity within BRO-2 all failed to support the hypothesis that
BRO-2 was more common in the past and has been selectively replaced
by BRO-1.
Keywords: disease transmission, molecular epidemiology,
selection
BRO
-lactamase alleles,
antibiotic resistance and a test of the BRO-1 selective replacement
hypothesis in Moraxella catarrhalis
2 Department of Internal
Medicine, Box 70622,
East Tennessee State University, Johnson City, TN 37614; James H. Quillen Veterans Affairs
Medical
Center (11C), Mountain Home, TN 37684, USA
-lactamase was
tested by examining the temporal distribution, antibiotic resistance
and epidemiological characteristics of isolates from a long-term
collection at a single locale.-lactamase-producing
isolates. Probes of dot blots were used to distinguish PCR failure
from non--lactamase-mediated penicillin
resistance.-lactamase producers exceeded the clinical
threshold for penicillin resistance. Bimodality of penicillin MICs
for -lactamase producers was caused
by variation within BRO-1 rather than differences between BRO-1
and BRO-2. Non--lactamase factors also
confer resistance to penicillin and may contribute to the BRO-1
bimodality. The 13 BRO-2 isolates were associated with diverse genotypes
within which there was evidence of epidemiologically linked clusters.
The exclusive association of BRO-2 with four unrelated genotypes
suggested maintenance of BRO-2 by recurrent mutation or horizontal
exchange.
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