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JAC Advance Access published online on November 12, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh017
© 2003 by The British Society for Antimicrobial Chemotherapy
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© 2003 The British Society for Antimicrobial Chemotherapy

Brief report

SHV-34: an extended-spectrum {beta}-lactamase encoded by an epidemic plasmid

John Heritage 1 *, Philip A. Chambers 1 , Caroline Tyndall 2 , and E. Stephen Buescher 2

1 Division of Microbiology, School of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, UK;
2 Center for Pediatric Research, Department of Pediatrics, Eastern Virginia Medical School, Children’s Hospital of The King’s Daughters, 855 West Brambleton Avenue, Norfolk, Virginia 23510-1001, USA

* Corresponding author. E-mail: j.heritage{at}leeds.ac.uk.

Received 21 May 2003 ; revised 6 October 2003 ; accepted 9 October 2003

Abstract

Objectives: To elucidate the causes for treatment failure in children given extended-spectrum cephalosporins.

Methods: During April 1998-March 2000, 18 isolates of members of the family Enterobacteriaceae, fulfilling microbiological criteria for carriage of extended-spectrum {beta}-lactamases (ESBLs) and carrying blaSHV, were isolated from paediatric inpatients. The collection was subjected to a retrospective molecular analysis.

Results: Three species were represented in the collection: Citrobacter koseri (one isolate), Escherichia coli (one isolate) and Klebsiella pneumoniae (16 isolates). A common plasmid was found in these bacteria, as judged by restriction endonuclease digestion. This was able to transfer an ESBL phenotype from donors to a laboratory strain of E. coli. Nucleotide sequence analysis revealed that this phenotype was associated with a new variant in blaSHV encoding SHV-34.

Conclusions: Analysis reveals the presence of an epidemic plasmid in this collection of bacteria. This carries a gene encoding the SHV-34 ESBL, described for the first time in this report. Nucleotide sequence analysis shows that there is a mutation from A->G affecting the codon at amino acid position 64 (GAA->GGA), changing the glutamic acid typically seen in this position to glycine.

Keywords: ESBLs, Enterobacteriaceae, SHV {beta}-lactamase
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