Skip Navigation



JAC Advance Access published online on November 12, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkh004
© 2003 by The British Society for Antimicrobial Chemotherapy
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
52/6/961    most recent
dkh004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Campos, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Campos, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2003 The British Society for Antimicrobial Chemotherapy

Original article

Antibiotic resistance and clinical significance of Haemophilus influenzae type f

José Campos 1 *, Federico Román 1 , María Pérez-Vázquez 1 , Belén Aracil 1 , Jesús Oteo 1 , Emilia Cercenado 2 , and and the Spanish Study Group for H. influenzae type f

1 Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ministry of Health, 28220 Majadahonda, Madrid;
2 Servicio de Microbiología, Hospital Gregorio Marañón, Madrid, Spain

* Corresponding author. E-mail: jcampos{at}isciii.es.

Received 4 July 2003 ; revised 30 September 2003 ; accepted 3 October 2003

Abstract

Objectives: Little is known about the antibiotic susceptibility and clinical significance of non-type b capsulated Haemophilus influenzae. We studied the antibiotic resistance patterns, plasmid carriage and clinical features of H. influenzae type f infections in Spain during 1996-2002.

Patients and methods: Forty-nine H. influenzae type f recovered from Spanish hospitals were analysed at a central laboratory where full microbiological and molecular epidemiological studies were carried out. Antimicrobial susceptibility testing was performed in accordance with NCCLS guidelines.

Results: Twelve strains (24.5%) were resistant to ampicillin and 22 (44.9%) to co-trimoxazole. Decreased susceptibility to clarithromycin, tetracycline, chloramphenicol and rifampicin was found in 16.3%, 12.2%, 14.3% and 2% of strains, respectively. Multidrug resistance was present in nine (18.4%) of the 49 isolates. The most prevalent resistance phenotype was ampicillin/tetracycline/co-trimoxazole/chloramphenicol, which was detected in five isolates. All six strains that were simultaneously resistant to ampicillin, tetracycline and chloramphenicol had conjugative plasmids. The main clinical diagnoses were pneumonia (32.6%), sepsis (18.4%) and meningitis (16.3%). Thirty-two patients (65.3%) had previous underlying predisposing conditions, principally respiratory diseases (20.4%). Twenty-one patients (42.8%) had impaired immunity. Thirty-seven (75.5%) patients were >14 years old, 12 (24.5%) were <=14 years, and seven were <=5 years. Most isolates were clonally related.

Conclusions: A high prevalence of antibiotic resistance, including multiresistance, was detected in Spanish H. influenzae type f isolates. Carriage of large conjugative plasmids was strongly associated with antibiotic resistance. H. influenzae type f is mainly an opportunistic pathogen, although it may cause primary severe infections, such as meningitis in children.

Keywords: H. influenzae, plasmid carriage, opportunistic infections
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
S. Garcia-Cobos, J. Campos, E. Cercenado, F. Roman, E. Lazaro, M. Perez-Vazquez, F. de Abajo, and J. Oteo
Antibiotic Resistance in Haemophilus influenzae Decreased, except for {beta}-Lactamase-Negative Amoxicillin-Resistant Isolates, in Parallel with Community Antibiotic Consumption in Spain from 1997 to 2007
Antimicrob. Agents Chemother., August 1, 2008; 52(8): 2760 - 2766.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
A. Jain, P. Kumar, and S. Awasthi
High ampicillin resistance in different biotypes and serotypes of Haemophilus influenzae colonizing the nasopharynx of healthy school-going Indian children
J. Med. Microbiol., February 1, 2006; 55(2): 133 - 137.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
B. Bruun, B. Gahrn-Hansen, H. Westh, and M. Kilian
Clonal relationship of recent invasive Haemophilus influenzae serotype f isolates from Denmark and the United States
J. Med. Microbiol., November 1, 2004; 53(11): 1161 - 1165.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
I. Z. Ecevit, K. W. McCrea, M. M. Pettigrew, A. Sen, C. F. Marrs, and J. R. Gilsdorf
Prevalence of the hifBC, hmw1A, hmw2A, hmwC, and hia Genes in Haemophilus influenzae Isolates
J. Clin. Microbiol., July 1, 2004; 42(7): 3065 - 3072.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
F. Roman, R. Canton, M. Perez-Vazquez, F. Baquero, and J. Campos
Dynamics of Long-Term Colonization of Respiratory Tract by Haemophilus influenzae in Cystic Fibrosis Patients Shows a Marked Increase in Hypermutable Strains
J. Clin. Microbiol., April 1, 2004; 42(4): 1450 - 1459.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.