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JAC Advance Access published online on September 30, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg439
© 2003 by The British Society for Antimicrobial Chemotherapy
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© 2003 The British Society for Antimicrobial Chemotherapy

Original article

Development of a microdilution method to evaluate Mycobacterium tuberculosis drug susceptibility

Elena Banfi 1 *, Giuditta Scialino 1 , and Carlo Monti-Bragadin 1

1 Dipartimento di Scienze Biomediche, sez. Microbiologia, Università degli Studi di Trieste, Trieste, Italy

* Corresponding author. E-mail: banfi{at}dsbmail.units.it.

Received 30 July 2002 ; revised 21 February 2003 ; accepted 9 August 2003

Abstract

A new rapid microdilution method, employing the dye resazurin as an indicator of mycobacterial growth, was developed to evaluate drug susceptibility of Mycobacterium tuberculosis reference strain H37Rv and of 13 M. tuberculosis susceptible or multidrug-resistant clinical strains. Different growth conditions were evaluated. The MICs of isoniazid, rifampicin, streptomycin and ethambutol were determined by the Microdilution Resazurin Assay (MRA) and the results compared with those obtained by the agar proportion method; complete agreement was always obtained. MRA resulted in a rapid, reliable, simple and inexpensive coloured method suitable for testing the susceptibility of M. tuberculosis clinical strains to first-line drugs; its employment in evaluating new antibacterial molecules is also suggested.

Keywords: M. tuberculosis, resazurin, MIC evaluation, microdilution assay, first-line antitubercular drugs
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