Skip Navigation



JAC Advance Access published online on September 1, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg416
© 2003 by The British Society for Antimicrobial Chemotherapy
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
52/4/699    most recent
dkg416v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Gales, A. C.
Right arrow Articles by Sader, H. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gales, A. C.
Right arrow Articles by Sader, H. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2003 The British Society for Antimicrobial Chemotherapy

Brief report

Dissemination in distinct Brazilian regions of an epidemic carbapenem-resistant Pseudomonas aeruginosa producing SPM metallo-{beta}-lactamase

Ana C. Gales 1 *, Liana C. Menezes 1 , Suzane Silbert 2 , and Hélio S. Sader 2

1 Laboratório Alerta; Laboratório Especial de Microbiologia Clínica, Division of Infectious Diseases, Universidade Federal de São Paulo, Rua Leandro Duprét, 188, São Paulo, SP - 04025-010, Brazil
2 Laboratório Especial de Microbiologia Clínica, Division of Infectious Diseases, Universidade Federal de São Paulo, Rua Leandro Duprét, 188, São Paulo, SP - 04025-010, Brazil

* Corresponding author. E-mail: galesac{at}aol.com.

Received 3 April 2003 ; revised 14 July 2003 ; accepted 15 July 2003

Abstract

Background: In Brazil, carbapenem use has been limited by high carbapenem-resistance rates among Pseudomonas aeruginosa isolates.

Objective: The main objective of this study was to evaluate the presence of an epidemic P. aeruginosa strain in unrelated Brazilian hospitals. We also aimed to search for the gene blaSPM, which encodes production of SPM, a novel metallo-{beta}-lactamase (MBL).

Methods: A reference broth microdilution method was used for antimicrobial susceptibility testing. The isolates were typed by ribotyping and pulsed-field gel electrophoresis (PFGE). A disc-approximation test using MBL inhibitors was employed to screen isolates for MBL production. PCR was used to search for the gene blaSPM.

Results: A total of 43 clinical isolates of carbapenem-resistant P. aeruginosa were collected from 12 hospitals. Colistin retained greatest activity in vitro. A single ribogroup included 17 P. aeruginosa isolates (39.5%) collected from seven unrelated hospitals located in five Brazilian states. Sixteen of these isolates showed an identical PFGE pattern, and 15 produced an SPM-1-like MBL. The remaining 26 isolates were grouped into 25 diverse ribogroups; none were MBL producers.

Conclusions: The emergence and dissemination of an epidemic clone has contributed to the high carbapenem resistance rates among P. aeruginosa isolates in Brazil. In addition, the production of SPM MBL has an important role in carbapenem resistance in this region. This is the first report of dissemination of an SPM-1-like-MBL-producing strain of P. aeruginosa among unrelated Brazilian hospitals.

Keywords: Gram-negative bacilli, carbapenem resistance, Brazil
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
P. D. Lister, D. J. Wolter, and N. D. Hanson
Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms
Clin. Microbiol. Rev., October 1, 2009; 22(4): 582 - 610.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
R. C. Picao, L. Poirel, A. C. Gales, and P. Nordmann
Diversity of {beta}-Lactamases Produced by Ceftazidime-Resistant Pseudomonas aeruginosa Isolates Causing Bloodstream Infections in Brazil
Antimicrob. Agents Chemother., September 1, 2009; 53(9): 3908 - 3913.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
D. M. Livermore
Has the era of untreatable infections arrived?
J. Antimicrob. Chemother., September 1, 2009; 64(suppl_1): i29 - i36.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
R. C. Picao, L. Poirel, A. C. Gales, and P. Nordmann
Further Identification of CTX-M-2 Extended-Spectrum {beta}-Lactamase in Pseudomonas aeruginosa
Antimicrob. Agents Chemother., May 1, 2009; 53(5): 2225 - 2226.
[Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. Castanheira, T. R. Fritsche, H. S. Sader, R. N. Jones, Y. Doi, D. de Oliveira Garcia, and D. L. Paterson
RmtD 16S RNA Methylase in Epidemiologically Unrelated SPM-1-Producing Pseudomonas aeruginosa Isolates from Brazil
Antimicrob. Agents Chemother., April 1, 2008; 52(4): 1587 - 1588.
[Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
Y. Doi, A. C. R. Ghilardi, J. Adams, D. de Oliveira Garcia, and D. L. Paterson
High Prevalence of Metallo-{beta}-Lactamase and 16S rRNA Methylase Coproduction among Imipenem-Resistant Pseudomonas aeruginosa Isolates in Brazil
Antimicrob. Agents Chemother., September 1, 2007; 51(9): 3388 - 3390.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
Y. Doi, D. de Oliveira Garcia, J. Adams, and D. L. Paterson
Coproduction of Novel 16S rRNA Methylase RmtD and Metallo-{beta}-Lactamase SPM-1 in a Panresistant Pseudomonas aeruginosa Isolate from Brazil
Antimicrob. Agents Chemother., March 1, 2007; 51(3): 852 - 856.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
R. E. Mendes, K. A. Kiyota, J. Monteiro, M. Castanheira, S. S. Andrade, A. C. Gales, A. C. C. Pignatari, and S. Tufik
Rapid Detection and Identification of Metallo-{beta}-Lactamase-Encoding Genes by Multiplex Real-Time PCR Assay and Melt Curve Analysis
J. Clin. Microbiol., February 1, 2007; 45(2): 544 - 547.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
C. G. Giske, B. Libisch, C. Colinon, E. Scoulica, L. Pagani, M. Fuzi, G. Kronvall, and G. M. Rossolini
Establishing Clonal Relationships between VIM-1-Like Metallo-{beta}-Lactamase-Producing Pseudomonas aeruginosa Strains from Four European Countries by Multilocus Sequence Typing
J. Clin. Microbiol., December 1, 2006; 44(12): 4309 - 4315.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
Y.-S. Yu, T.-T. Qu, J.-Y. Zhou, J. Wang, H.-Y. Li, and T. R. Walsh
Integrons Containing the VIM-2 Metallo-{beta}-Lactamase Gene among Imipenem-Resistant Pseudomonas aeruginosa Strains from Different Chinese Hospitals
J. Clin. Microbiol., November 1, 2006; 44(11): 4242 - 4245.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. R. Marra, C. A. P. Pereira, A. C. Gales, L. C. Menezes, R. G. R. Cal, J. M. A. de Souza, M. B. Edmond, C. Faro, and S. B. Wey
Bloodstream Infections with Metallo-{beta}-Lactamase-Producing Pseudomonas aeruginosa: Epidemiology, Microbiology, and Clinical Outcomes
Antimicrob. Agents Chemother., January 1, 2006; 50(1): 388 - 390.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
A. P. Zavascki, P. B. Gaspareto, A. F. Martins, A. L. Goncalves, and A. L. Barth
Outbreak of carbapenem-resistant Pseudomonas aeruginosa producing SPM-1 metallo-{beta}-lactamase in a teaching hospital in southern Brazil
J. Antimicrob. Chemother., December 1, 2005; 56(6): 1148 - 1151.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
S. A. Nouer, M. Nucci, M. P. de-Oliveira, F. L. P. C. Pellegrino, and B. M. Moreira
Risk Factors for Acquisition of Multidrug-Resistant Pseudomonas aeruginosa Producing SPM Metallo-{beta}-Lactamase
Antimicrob. Agents Chemother., September 1, 2005; 49(9): 3663 - 3667.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
J. D. D. Pitout, D. B. Gregson, L. Poirel, J.-A. McClure, P. Le, and D. L. Church
Detection of Pseudomonas aeruginosa Producing Metallo-{beta}-Lactamases in a Large Centralized Laboratory
J. Clin. Microbiol., July 1, 2005; 43(7): 3129 - 3135.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
T. R. Walsh, M. A. Toleman, L. Poirel, and P. Nordmann
Metallo-{beta}-Lactamases: the Quiet before the Storm?
Clin. Microbiol. Rev., April 1, 2005; 18(2): 306 - 325.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
N. Lincopan, J. A. McCulloch, C. Reinert, V. C. Cassettari, A. C. Gales, and E. M. Mamizuka
First Isolation of Metallo-{beta}-Lactamase-Producing Multiresistant Klebsiella pneumoniae from a Patient in Brazil
J. Clin. Microbiol., January 1, 2005; 43(1): 516 - 519.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. P. Crespo, N. Woodford, A. Sinclair, M. E. Kaufmann, J Turton, J. Glover, J. D. Velez, C. R. Castaneda, M. Recalde, and D. M. Livermore
Outbreak of Carbapenem-Resistant Pseudomonas aeruginosa Producing VIM-8, a Novel Metallo-{beta}-Lactamase, in a Tertiary Care Center in Cali, Colombia
J. Clin. Microbiol., November 1, 2004; 42(11): 5094 - 5101.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
H. Nishio, M. Komatsu, N. Shibata, K. Shimakawa, N. Sueyoshi, T. Ura, K. Satoh, M. Toyokawa, T. Nakamura, Y. Wada, et al.
Metallo-{beta}-Lactamase-Producing Gram-Negative Bacilli: Laboratory-Based Surveillance in Cooperation with 13 Clinical Laboratories in the Kinki Region of Japan
J. Clin. Microbiol., November 1, 2004; 42(11): 5256 - 5263.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.