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JAC Advance Access published online on September 12, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg415
© 2003 by The British Society for Antimicrobial Chemotherapy
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© 2003 The British Society for Antimicrobial Chemotherapy

Original article

Evaluation of Oxoid combination discs for detection of extended-spectrum {beta}-lactamases

Yves De Gheldre 1 *, Véronique Avesani 2 , Catherine Berhin 1 , Michel Delmée 2 , and Youri Glupczynski 1

1 Department of Microbiology, Catholic University of Louvain, Mont-Godinne University Hospital, Yvoir;
2 Department of Microbiology, Saint-Luc University Hospital, Brussels, Belgium

* Corresponding author. E-mail: yves.degheldre{at}mont.ucl.ac.be.

Received 5 May 2003 ; revised 10 July 2003 ; accepted 15 July 2003

Abstract

Objectives: We evaluated the reliability of cefpirome/clavulanate (CD04) compared with ceftazidime/clavulanate (CD02) and cefotaxime/clavulanate (CD03) Oxoid combination discs for the detection of extended-spectrum {beta}-lactamases (ESBL) in several Enterobacteriaceae isolates, including Enterobacter spp.

Methods: Overall, a total of 105 ESBL-positive [positive double-disc synergy test (DDST)] and 94 ESBL-negative (negative DDST) Gram-negative isolates were evaluated. Ninety-eight isolates were confirmed as ESBL-positive on the basis of the sequence alignments of the blaTEM and/or blaSHV gene amplification products, which matched with previously identified ESBLs. The phenotypic detection of ESBLs was performed by the three combination discs according to the NCCLS and BSAC methods. The CD04 disc was evaluated with the manufacturer's recommended zone size difference breakpoint of >=4 mm.

Results: In Escherichia coli and Klebsiella spp., the sensitivities (%)/specificities (%) of CD02, CD03 and CD04 discs, and the combination of CD02 or CD04 discs, were, respectively, 88/92, 90/92, 95/84 and 100/82, while the corresponding figures were 94/100, 4/100, 94/100 and 100/100 in Enterobacter aerogenes. NCCLS and BSAC methods yielded concordant results in 99% of the isolates.

Conclusions: CD04 and CD02 discs were the best combination for detection of ESBLs in our collection of Enterobacteriaceae isolates, including E. aerogenes.

Keywords: Enterobacter aerogenes, ESBL detection, PCR sequencing, TEM, SHV
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