JAC Advance Access published online on September 1, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg410
© 2003 by The British Society for Antimicrobial Chemotherapy
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Original article
1 Department of Pathology
and Microbiology, University of Bristol, Bristol BS8 1TD, UK
* Corresponding author. E-mail: Mark.Toleman{at}bristol.ac.uk.
Received 23 May 2003
; revised 8 July 2003
; accepted 9 July 2003
Objective: In 2001, as part of the
SENTRY worldwide antimicrobial surveillance programme, Pseudomonas aeruginosa 86-14571A
was isolated at a hospital in Rome from a cancer patient with a
bloodstream infection. The isolate was resistant to all antibiotics
except amikacin, and displayed an imipenem MIC of 64 mg/L that decreased
to 8 mg/L in the presence of EDTA. The resistance determinant was
investigated. Methods: The resistant determinant was cloned
in Escherichia coli using a shotgun cloning approach. Results: Sequence analysis revealed the presence
of a novel IMP-type metallo- Conclusions: The resistance determinant of P. aeruginosa 86-14571A is a novel IMP-type
MBL carried on a composite transposon responsible for wide geographical
dissemination of MBL genes in Europe.
Keywords: metallo-Genetic characterization of a novel metallo-
-lactamase gene, blaIMP-13, harboured
by a novel Tn5051-type transposon disseminating carbapenemase
genes in Europe: report from the SENTRY worldwide antimicrobial
surveillance programme
2 The JONES Group/JMI Laboratories,
North Liberty, Iowa
3 The JONES Group/JMI Laboratories,
North Liberty, Iowa; Tufts
University School of Medicine, Boston, Massachusetts, USA
-lactamase
(MBL) gene, blaIMP-13. This encoded
a protein displaying most identity to IMP variants: 93% and
92.3% identity, respectively, to IMP-8 and IMP-2 (previously
identified in Italy). The protein had 19 amino acid changes from
IMP-2 and 17 amino acid changes from IMP-8. The blaIMP-13 gene
was found as a gene cassette in the first position of a class 1
integron. A 25 bp inverted repeat sequence IRi was identified 174
bp upstream of the class I integrase, which suggests that the integron
is found on a Tn402-like transposon, or defective
transposon derivative. This element, in turn, is located in the
transposition locus (tnp region) of a Tn21 subfamily
transposon that showed most identity to Tn5051,
a transposon recently identified from a strain of Pseudomonas
putida isolated in New York. Interestingly, the insertion point
of the Tn402-like transposon and the sequence of
the Tn5051-like genes were identical to those of
the genetic element harbouring blaVIM-2 recently
identified in Poland.-lactamases, Pseudomonas aeruginosa, integron Tn21,
Tn402
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