JAC Advance Access published online on September 1, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg395
© 2003 by The British Society for Antimicrobial Chemotherapy
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Brief report
1 Laboratorio Nacional de
Referencia de Haemophilus, Instituto de Medicina Tropical 'Pedro Kouri', La Habana, Cuba
* Corresponding author. E-mail: jcampos{at}isciii.es.
Received 31 October 2002
; revised 3 April 2003
; accepted 29 June 2003
Objectives: A national surveillance
study to determine antimicrobial susceptibility in Haemophilus
influenzae type b isolated from cerebrospinal fluid was carried
out in Cuba from 1990 to 2002. Methods: Susceptibility to ampicillin, co-amoxiclav,
cefotaxime, ceftriaxone, co-trimoxazole, tetracycline, chloramphenicol
and rifampicin was tested by the microdilution method according
to the NCCLS guidelines. Results: The 34 participating laboratories recovered
938 consecutive, non-identical isolates. All the isolates were retrieved
from children aged <5 years. The mean number of isolates
collected by year in the pre-vaccination era (1990-1998)
was 93; after vaccination, 57 isolates were reported in 1999, 31
in 2000, four in 2001 and five in 2002. Resistance to ampicillin,
co-trimoxazole, tetracycline and chloramphenicol was 46.3% (all Conclusions: In Cuba, the widespread vaccination
against Haemophilus influenzae type b prevented
a large number of meningitis cases in children caused by strains
resistant to multiple antibiotics.
Keywords: Haemophilus influenzae type
b, antimicrobial resistance, meningitis, conjugate vaccines
High levels of multiple antibiotic resistance among
938
Haemophilus influenzae type b meningitis isolates from Cuba
(1990-2002)
2 Centro Nacional de Microbiología,
Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Carretera
Pozuelo a Majadahonda, 28220. Majadahonda, Madrid, Spain
-lactamase-positive), 51.3%,
33.2% and 44.0%, respectively. Ampicillin-resistant -lactamase-negative strains were not
detected. All strains were susceptible to co-amoxiclav, cefotaxime,
ceftriaxone and rifampicin. Ampicillin resistance was strongly associated
with resistance to tetracycline, co-trimoxazole and chloramphenicol
(P < 0.001). Multidrug resistance was
present in 43.8% of isolates. The most prevalent phenotype
was resistance to ampicillin/chloramphenicol/tetracycline/co-trimoxazole,
which was detected in 29.2% of strains overall. An increase
in the prevalence of resistance to these antibiotics was observed from
1990 to 2000 in the range 40.7%-54.8% for
ampicillin, 40.1%-51.6% for chloramphenicol,
45.4%-58.1% for co-trimoxazole and 23%-45.2% for
tetracycline.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Skoczynska, M. Kadlubowski, J. Empel, and W. Hryniewicz Characteristics of Haemophilus influenzae Type b Responsible for Meningitis in Poland from 1997 to 2004 J. Clin. Microbiol., November 1, 2005; 43(11): 5665 - 5669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Oteo, E. Lazaro, F. J. de Abajo, F. Baquero, J. Campos, and Spanish Members of the European Antimicrobial Resi Trends in Antimicrobial Resistance in 1,968 Invasive Streptococcus pneumoniae Strains Isolated in Spanish Hospitals (2001 to 2003): Decreasing Penicillin Resistance in Children's Isolates J. Clin. Microbiol., December 1, 2004; 42(12): 5571 - 5577. [Abstract] [Full Text] [PDF]
|
|