JAC Advance Access published online on September 1, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg390
© 2003 by The British Society for Antimicrobial Chemotherapy
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Brief report
1 Department of Microbiology, Faculty of Pharmaceutical Sciences,
Okayama University, Tsushima, Okayama, 700-8530, Japan
* Corresponding author. E-mail: tsuchiya{at}pharm.okayama-u.ac.jp.
Received 30 April 2003
; revised 19 June 2003
; accepted 25 June 2003
Objectives: Multidrug efflux pumps
are thought to be involved in mediating multidrug resistance in Pseudomonas aeruginosa. Here we aim to characterize
hitherto uncharacterized multidrug efflux pumps from
P. aeruginosa. Materials and methods: We isolated a mutant,
YM442, which showed elevated resistance to several antimicrobial
agents from P. aeruginosa YM44 lacking four major
multidrug efflux pumps, MexAB, MexCD-OprJ, MexEF-OprN
and MexXY. We cloned genes responsible for the resistance from chromosomal
DNA of YM442 using YM44 as host. Results: We designated the genes mexVW.
Introduction of a recombinant plasmid pTAJ2 carrying the mexVW into
YM44 cells conferred resistance to fluoroquinolones, tetracycline,
chloramphenicol, erythromycin, ethidium bromide and acriflavine.
Elevated ethidium bromide extrusion was observed with cells of YM442
and of YM44/pTAJ2. An outer membrane protein OprM was able to cooperate
with MexVW. Elevated expression of the mexV gene
was observed with YM442 compared with YM44. Conclusions: MexV (membrane fusion protein)-MexW
(RND-type membrane protein)-OprM is a tripartite multidrug
efflux pump. It is suggested that other outer membrane component(s)
could cooperate with MexVW.
Keywords: MexVW, multidrug efflux pump, RND-type, P.
aeruginosa
A new member of the tripartite multidrug efflux
pumps,
MexVW-OprM, in Pseudomonas aeruginosa
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Mima, N. Kohira, Y. Li, H. Sekiya, W. Ogawa, T. Kuroda, and T. Tsuchiya Gene cloning and characteristics of the RND-type multidrug efflux pump MuxABC-OpmB possessing two RND components in Pseudomonas aeruginosa Microbiology, November 1, 2009; 155(11): 3509 - 3517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. D. Lister, D. J. Wolter, and N. D. Hanson Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms Clin. Microbiol. Rev., October 1, 2009; 22(4): 582 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Strateva and D. Yordanov Pseudomonas aeruginosa - a phenomenon of bacterial resistance J. Med. Microbiol., September 1, 2009; 58(9): 1133 - 1148. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Vettoretti, P. Plesiat, C. Muller, F. El Garch, G. Phan, I. Attree, A. Ducruix, and C. Llanes Efflux Unbalance in Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients Antimicrob. Agents Chemother., May 1, 2009; 53(5): 1987 - 1997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Jeannot, S. Elsen, T. Kohler, I. Attree, C. van Delden, and P. Plesiat Resistance and Virulence of Pseudomonas aeruginosa Clinical Strains Overproducing the MexCD-OprJ Efflux Pump Antimicrob. Agents Chemother., July 1, 2008; 52(7): 2455 - 2462. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhang and T.-F. Mah Involvement of a Novel Efflux System in Biofilm-Specific Resistance to Antibiotics J. Bacteriol., July 1, 2008; 190(13): 4447 - 4452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Struble and R. T. Gill Reverse Engineering Antibiotic Sensitivity in a Multidrug-Resistant Pseudomonas aeruginosa Isolate. Antimicrob. Agents Chemother., July 1, 2006; 50(7): 2506 - 2515. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Pumbwe, O. Ueda, F. Yoshimura, A. Chang, R. L. Smith, and H. M. Wexler Bacteroides fragilis BmeABC efflux systems additively confer intrinsic antimicrobial resistance J. Antimicrob. Chemother., July 1, 2006; 58(1): 37 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hocquet, P. Nordmann, F. El Garch, L. Cabanne, and P. Plesiat Involvement of the MexXY-OprM Efflux System in Emergence of Cefepime Resistance in Clinical Strains of Pseudomonas aeruginosa. Antimicrob. Agents Chemother., April 1, 2006; 50(4): 1347 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Alyaseen, K. E. Piper, M. S. Rouse, J. M. Steckelberg, and R. Patel Selection of Cross-Resistance following Exposure of Pseudomonas aeruginosa Clinical Isolates to Ciprofloxacin or Cefepime Antimicrob. Agents Chemother., June 1, 2005; 49(6): 2543 - 2545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Sobel, S. Neshat, and K. Poole Mutations in PA2491 (mexS) Promote MexT-Dependent mexEF-oprN Expression and Multidrug Resistance in a Clinical Strain of Pseudomonas aeruginosa J. Bacteriol., February 15, 2005; 187(4): 1246 - 1253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kriengkauykiat, E. Porter, O. Lomovskaya, and A. Wong-Beringer Use of an Efflux Pump Inhibitor To Determine the Prevalence of Efflux Pump-Mediated Fluoroquinolone Resistance and Multidrug Resistance in Pseudomonas aeruginosa Antimicrob. Agents Chemother., February 1, 2005; 49(2): 565 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Nehme, X.-Z. Li, R. Elliot, and K. Poole Assembly of the MexAB-OprM Multidrug Efflux System of Pseudomonas aeruginosa: Identification and Characterization of Mutations in mexA Compromising MexA Multimerization and Interaction with MexB J. Bacteriol., May 15, 2004; 186(10): 2973 - 2983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Middlemiss and K. Poole Differential Impact of MexB Mutations on Substrate Selectivity of the MexAB-OprM Multidrug Efflux Pump of Pseudomonas aeruginosa J. Bacteriol., March 1, 2004; 186(5): 1258 - 1269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-X. He, T. Kuroda, T. Mima, Y. Morita, T. Mizushima, and T. Tsuchiya An H+-Coupled Multidrug Efflux Pump, PmpM, a Member of the MATE Family of Transporters, from Pseudomonas aeruginosa J. Bacteriol., January 1, 2004; 186(1): 262 - 265. [Abstract] [Full Text] [PDF]
|
|