JAC Advance Access published online on September 1, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg388
© 2003 by The British Society for Antimicrobial Chemotherapy
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Brief report
1 University Hospital Virgen
Macarena, Seville
* Corresponding author. E-mail: jmrodriguez{at}us.es.
Received 5 March 2003
; revised 25 June 2003
; accepted 25 June 2003
Objectives: Plasmid pMG252 contains
the qnr locus, which is responsible for low-level
resistance to quinolones by protecting the DNA gyrase. pMG252 also
encodes the AmpC-type Methods: Four hundred and twenty-five (159 Klebsiella pneumoniae and 266 Escherichia
coli) clinical isolates were studied. The detection of qnr was by
PCR using specific primers for an internal fragment of 543 bp. Results: qnr was detected in
three cefoxitin-resistant K. pneumoniae strains,
which also produced a pACBL. None of the E. coli isolates
tested contained qnr. The three qnr-positive K. pneumoniae came from the USA, and all transferred
a conjugative plasmid coding for cefoxitin resistance to E.
coli J53. qnr was also transferred by the
same plasmid in two out of the three strains. The sequences of amplified qnr fragments from the three strains were identical
to the qnr sequence from pMG252. Conclusions: The qnr determinant
is uncommon among clinical isolates of K. pneumoniae and E. coli, but its identification in three pACBL(+) K. pneumoniae from the USA indicates the emergence
of this quinolone resistance mechanism.
Keywords: Escherichia coli, fluoroquinolones,
cephamycinase, molecular epidemiology
Detection of the plasmid-mediated quinolone resistance
determinant qnr among clinical isolates of Klebsiella
pneumoniae producing
AmpC-type
-lactamase
2 University Hospital Virgen
Macarena and School of Medicine,
University of Seville, Seville, Spain
-lactamase (pACBL),
FOX-5. The aim of this study was to determine the prevalence of qnr in strains from different geographical locations
in America and Europe.
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