Skip Navigation



JAC Advance Access published online on August 13, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg384
© 2003 by The British Society for Antimicrobial Chemotherapy
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
52/3/365    most recent
dkg384v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Martín-Mazuelos, E.
Right arrow Articles by Cantón, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martín-Mazuelos, E.
Right arrow Articles by Cantón, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2003 The British Society for Antimicrobial Chemotherapy

Original article

Comparison of the Sensititre YeastOne colorimetric antifungal panel and Etest with the NCCLS M38-A method to determine the activity of amphotericin B and itraconazole against clinical isolates of Aspergillus spp

Estrella Martín-Mazuelos 1 , Javier Pemán 2 , Anastasio Valverde 1 , Mónica Chaves 1 , M. Carmen Serrano 1 , and Emilia Cantón 3 *

1 Servicio de Microbiología, Hospital Universitario de Valme, Sevilla;
2 Servicio de Microbiología, Hospital Universitario La Fe, Valencia;
3 Unidad de Bacteriología Experimental, Centro de Investigación, Hospital Universitario La Fe, Avenida Campanar 21, 46009 Valencia, Spain

* Corresponding author. E-mail: canton_emi{at}gva.es.

Received 31 March 2003 ; revised 20 June 2003 ; accepted 21 June 2003

Abstract

Objective: The evaluation of the Sensititre YeastOne and Etest methods for determining susceptibility to amphotericin B and itraconazole by comparing the MICs obtained by these methods at different times of reading with those of the M38-A broth microdilution method.

Methods: Sixty-three clinical isolates of Aspergillus spp. (23 Aspergillus flavus, 24 Aspergillus fumigatus, nine Aspergillus niger, three Aspergillus glaucus, two Aspergillus terreus and two Aspergillus flavipes) were assayed. Two itraconazole-resistant strains (NCPF7100 and 7099) were also included.

Results: Itraconazole MICs for the two resistant strains were >4 mg/L by the three methods. The overall agreement (±2 log2) between M38-A (48 h) and colorimetric (48 h) method was 93.4% for amphotericin B and 90.2% for itraconazole. By the Etest, the best agreement with M38-A was obtained when readings were made at 24 h: 88.5% for amphotericin B and 67.2% for itraconazole. Etest MICs were higher for all species except A. niger.

Conclusions: The colorimetric method appears to be a suitable alternative procedure for antifungal susceptibility testing of Aspergillus spp. and is able to detect resistance to itraconazole. The range of MICs for amphotericin B by Etest is wider and for some strains is >16 mg/L, suggesting that this method could be useful for detecting resistant strains as occur in yeasts.

Keywords: Aspergillus spp., susceptibility testing, MIC, Sensititre YeastOne
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
E. Alvarado-Ramirez and J. M. Torres-Rodriguez
In Vitro Susceptibility of Sporothrix schenckii to Six Antifungal Agents Determined Using Three Different Methods
Antimicrob. Agents Chemother., July 1, 2007; 51(7): 2420 - 2423.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
A. Espinel-Ingroff
Comparison of Three Commercial Assays and a Modified Disk Diffusion Assay with Two Broth Microdilution Reference Assays for Testing Zygomycetes, Aspergillus spp., Candida spp., and Cryptococcus neoformans with Posaconazole and Amphotericin B
J. Clin. Microbiol., October 1, 2006; 44(10): 3616 - 3622.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
W. W. J. van de Sande, A. Luijendijk, A. O. A. Ahmed, I. A. J. M. Bakker-Woudenberg, and A. van Belkum
Testing of the In Vitro Susceptibilities of Madurella mycetomatis to Six Antifungal Agents by Using the Sensititre System in Comparison with a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5- [(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Assay and a Modified NCCLS Method
Antimicrob. Agents Chemother., April 1, 2005; 49(4): 1364 - 1368.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
E. Canton, J. Peman, M. Gobernado, E. Alvarez, F. Baquero, R. Cisterna, J. Gil, E. Martin-Mazuelos, C. Rubio, A. Sanchez-Sousa, et al.
Sensititre YeastOne Caspofungin Susceptibility Testing of Candida Clinical Isolates: Correlation with Results of NCCLS M27-A2 Multicenter Study
Antimicrob. Agents Chemother., April 1, 2005; 49(4): 1604 - 1607.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
C. Castro, M. C. Serrano, B. Flores, A. Espinel-Ingroff, and E. Martin-Mazuelos
Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with a Modified NCCLS M38-A Method To Determine the Activity of Voriconazole against Clinical Isolates of Aspergillus spp.
J. Clin. Microbiol., September 1, 2004; 42(9): 4358 - 4360.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.