JAC Advance Access published online on August 13, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg379
© 2003 by The British Society for Antimicrobial Chemotherapy
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Brief report
1 Veterinary Laboratories
Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB
* Corresponding author. E-mail: l.randall{at}vla.defra.gsi.gov.uk.
Received 16 April 2003
; revised 16 June 2003
; accepted 17 June 2003
Aims: In view of recent findings that
a multidrug efflux pump CmeABC exists in Campylobacter
jejuni, 391 C. jejuni and 52 Campylobacter
coli of human and animal origin were examined for a multidrug
resistance phenotype. Materials and methods: The MICs of ampicillin,
chloramphenicol, ciprofloxacin, erythromycin, kanamycin, tetracycline,
cetrimide, triclosan, acridine orange, paraquat and ethidium bromide
were determined. Resistance to organic solvents and the effect of
salicylate (known inducer of the marRAB operon
in Escherichia coli and Salmonella)
were also examined. Results: Two C. coli and 13 C. jejuni isolates, mainly from pigs or poultry,
were resistant to three or more antibiotics and 12 of these strains
had reduced susceptibility to acridine orange and/or ethidium bromide. Strains
(n = 20) that were less susceptible to
acridine orange, ethidium bromide and triclosan were significantly
more resistant (P < 0.05) to ampicillin,
chloramphenicol, ciprofloxacin, erythromycin, nalidixic acid and
tetracycline, with two- to four-fold increases in MIC values compared
with strains (n = 20) most susceptible
to acridine orange, ethidium bromide and triclosan. Growth of strains
with 1 mM salicylate caused a small (up to two-fold) but statistically
significant (P Conclusions: These data indicate that multiple
antibiotic resistant (MAR)-like Campylobacter strains
occur and it may be postulated that these may overexpress cmeABC or
another efflux system.
Keywords: efflux, cmeABC, salicylate,
ethidium bromide
Prevalence of multiple antibiotic resistance in
443 Campylobacter spp. isolated from humans and
animals
2 Antimicrobial Agents Research
Group, Division of Immunity and Infection, The Medical School, University
of Birmingham, Edgbaston, Birmingham B15 2TT, UK
0.005)
increase in the MICs of chloramphenicol, ciprofloxacin, erythromycin
and tetracycline.
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