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JAC Advance Access published online on July 29, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg344
© 2003 by The British Society for Antimicrobial Chemotherapy
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© 2003 The British Society for Antimicrobial Chemotherapy

Brief report

Partial characterization of a transposon containing the tet(A) determinant in a clinical isolate of Acinetobacter baumannii

Anna Ribera 1 , Ignasi Roca 2 , Joaquim Ruiz 1 , Isidre Gibert 2 , and Jordi Vila 1 *

1 Servei de Microbiologia, Institut Clinic Infeccions i Immunologia, IDIBAPS, Facultat de Medicina, Universitat de Barcelona, Villarroel, 170, 08036 Barcelona
2 Laboratori de Genètica Molecular Bacteriana, Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain

* Corresponding author. E-mail: vila{at}medicina.ub.es.

Received 10 May 2002 ; revised 16 January 2003 ; accepted 18 May 2003

Abstract

A genomic library from one clinical isolate of Acinetobacter baumannii was obtained to find genes responsible for tetracycline resistance. Escherichia coli DH5{alpha}-MDR transformants, selected on Mueller-Hinton agar supplemented with tetracycline 18 mg/L, were thoroughly characterized. In one clone, with an insert of 7070 bp, it was found that the resistance to tetracycline was mediated by the tet(A) gene (1200 bp) which encodes a tetracycline efflux pump. This gene was recovered together with tetR(A) (651 bp), the tet(A) repressor. Moreover, the partial sequence (2008 bp) of a transposase gene, tnpA, and 1316 bp corresponding to an IS, similar to that described in one strain of Salmonella typhi (IS4321), were found. In this A. baumannii clinical isolate, the tet(A) gene is located in a transposon. The structure of this transposon is similar to that of Tn1721, with the tet(A), tetR(A) and the regions between these genes, being closely related to those of Tn1721. The data indicate horizontal transfer of tetracycline resistance genes between A. baumannii and other genera sharing the same ecological niches.

Keywords: tetracycline resistance, Acinetobacter baumannii
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