JAC Advance Access published online on July 15, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg336
© 2003 by The British Society for Antimicrobial Chemotherapy
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Brief report
1 Microbiology Group, Instituto
Nacional de Salud, Avenida Calle 26 No 51-60, Bogotá;
* Corresponding author. E-mail: mhidalgo{at}ins.gov.co.
Received 20 January 2003
; revised 5 May 2003
; accepted 15 May 2003
The present study was set up to determine vancomycin
tolerance in Colombian, multiresistant, invasive, paediatric isolates
of Streptococcus pneumoniae. A total of seven clinical
isolates of penicillin-resistant S. pneumoniae,
serotype 14, corresponding to the Spain9v-3 clone, were
studied. Isolates were grown in Todd Hewitt broth
at 37°C. Growth (A595)
and cell viability (cfu/mL) were monitored hourly for 4 h after
addition of vancomycin at 10x MIC. S. pneumoniae R6 was used as a control for growth.
For R6, the mean decrease in A595 and
cell viability (log10 cfu/mL) after 4 h of vancomycin
treatment (in 10 independent experiments) was 95.1% and
4.2 log10, respectively. Six out of seven isolates exhibited
similar behaviour. In contrast, for S. pneumoniae E239,
the mean decrease in A595 and cell viability
was 48.5% and 2.2 log10 after 4 h of
vancomycin exposure, which suggested the presence of tolerance to
vancomycin. The emergence of vancomycin-tolerant strains is of great
concern in Colombia, owing to the alarming increase in resistance
to penicillin.
Keywords: antimicrobial tolerance, vancomycin, Colombian
isolates, killing curves, Streptococcus pneumoniae
Tolerance to vancomycin in a multiresistant, Colombian
isolate of Streptococcus pneumoniae
2 Bacterial Molecular Genetics Unit,
Centro de Investigaciones, Universidad El Bosque, Bogotá,
D.C., Colombia
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