JAC Advance Access published online on June 12, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg300
© 2003 by The British Society for Antimicrobial Chemotherapy
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Brief report
1 Microbiology and Clinical
Microbiology Department of Fatih University School of Medicine,
Ankara
* Corresponding author. E-mail: zcacikgoz{at}yahoo.com.
Received 4 January 2003
; revised 11 February 2003
; accepted 23 April 2003
Objectives: Macrolide-lincosamide-streptogramin
B (MLSB) resistance determinants of group A streptococci
(GAS) in Ankara, Turkey, were defined for the first time. Isolates and methods: A total of 1355 GAS isolates,
collected from three different regions of Ankara, were screened
for erythromycin resistance. Resistance phenotypes were determined
by a triple-disc test, and the gene determinants responsible
were determined by PCR. MICs of erythromycin, clindamycin and spiramycin
were measured for the resistant isolates, and susceptibility rates
to some further antibiotics were determined. Results: Thirty-six isolates (2.6%)
were resistant to erythromycin. Of these, 17 (47.2%) expressed
macrolide-restricted resistance (M phenotype), while the remainder
expressed inducible (16 isolates, 44.4%) or constitutive
(three isolates, 8.3%) MLSB resistance. All
isolates of the M phenotype harboured the mef(A) gene.
Of non-M isolates, 14 harboured erm(A) subclass erm(TR) and five had erm(B) genes.
There was a significant relationship between tetracycline resistance
and the inducible phenotype (P < 0.05).
Macrolide resistance was significantly higher in adults (P < 0.05),
and increased more than two-fold in 2002 compared with 2001 (P < 0.05). Conclusion: The prevalence of macrolide resistance
in GAS is low in Ankara; therefore, routine antimicrobial susceptibility
testing against these agents seems unwarranted.
Keywords: Streptococcus pyogenes, macrolide-lincosamide-streptogramin
B resistance, MLSB
Macrolide resistance determinants of group A streptococci
in Ankara, Turkey
2 METIS Biotechnology
Ltd, Ankara, Turkey
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