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JAC Advance Access published online on June 12, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg268
© 2003 by The British Society for Antimicrobial Chemotherapy
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© 2003 The British Society for Antimicrobial Chemotherapy

Original article

Effect of chloramphenicol, erythromycin, moxifloxacin, penicillin and tetracycline concentration on the recovery of resistant mutants of Mycobacterium smegmatis and Staphylococcus aureus

Tao Lu 1 , Xilin Zhao 1 , Xinying Li 1 , Glen Hansen 2 , Joseph Blondeau 2 , and Karl Drlica 1 *

1 Public Health Research Institute, 225 Warren St., Newark, NJ 07103, USA
2 Departments of Clinical Microbiology, St. Paul's Hospital (Grey Nuns') and Saskatoon and District Health; Department of Pathology, Royal University Hospital and the Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

* Corresponding author. E-mail: drlica{at}phri.org.

Received 11 November 2002 ; revised 4 March 2003 ; accepted 2 April 2003

Abstract

The effect of antimicrobial concentration on colony-forming ability of resistant mutant subpopulations of Mycobacterium smegmatis and Staphylococcus aureus was measured for chloramphenicol, erythromycin, moxifloxacin, penicillin and tetracycline. The relationship between drug concentration and the recovery of mutant colonies was distinct for each bacterium-antimicrobial combination; however, in each case application of large numbers of cells to drug-containing agar plates revealed a progressive reduction in mutant recovery as antimicrobial concentration increased. The minimal concentration that allowed no mutant recovery from more than 1010 input cells was measured to estimate the minimum inhibitory concentration (MIC) of the least susceptible, single-step mutant subpopulation, a parameter also called the mutant prevention concentration (MPC). These data expand the number of antimicrobial-bacterial combinations for which a mutant selection window can be measured.

Keywords: erythromycin, moxifloxacin, penicillin, tetracycline, chloramphenicol
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