JAC Advance Access published online on May 29, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg256
© 2003 by The British Society for Antimicrobial Chemotherapy
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Original article
1 Laboratoire de Bactériologie,
Faculté de Médecine, Service de Bactériologie-Virologie,
28 Place Henri-Dunant, 63001 Clermont-Ferrand Cedex
* Corresponding author. E-mail: Richard.Bonnet{at}u-clermont1.fr.
Received 27 January 2003
; revised 13 March 2003
; accepted 13 March 2003
Escherichia coli clinical strain Gre-1
collected in 2000 from a French hospital harboured a novel CTX-M-encoding
gene, designated blaCTX-M-27. CTX-M-27
differed from CTX-M-14 only by the substitution D240G and was the
third CTX-M enzyme harbouring this mutation after CTX-M-15 and CTX-M-16.
The Gly-240-harbouring enzyme CTX-M-27 conferred to E.
coli higher MICs of ceftazidime (MIC, 8 versus 1 mg/L) than
did the Asp-240-harbouring CTX-M-14 enzyme. Comparison of CTX-M-14
and CTX-M-27 showed that residue Gly-240 decreased Km for
ceftazidime (205 versus 940 µM), but
decreased hydrolytic activity against good substrates, such as cefotaxime
(kcat, 113 versus 415 s-1),
probably owing to the alteration of
Keywords: CTX-M, Effect of D240G substitution in a novel ESBL CTX-M-27
2 Laboratoire de Bactériologie,
CHU de Grenoble,
Chemin Maquis du Grésivaudan, 38 700 La Tronche, France
3
strand positioning during the catalytic process.-lactamase,
D240G mutation, ESBL
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