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JAC Advance Access published online on April 14, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg221
© 2003 by The British Society for Antimicrobial Chemotherapy
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© 2003 The British Society for Antimicrobial Chemotherapy

Brief report

Sequence analysis of cfxA2-like {beta}-lactamases in Prevotella species

Chantal Giraud-Morin 1, Isabelle Madinier 2, Thierry Fosse 1*

1 Laboratoire de Bactériologie, Hôpital l'Archet 2, Centre Hospitalier Universitaire, 151 route de Saint Antoine Ginestière, 06202 Nice Cedex 3
2 Laboratoire de Bactériologie, Hôpital l'Archet 2, Centre Hospitalier Universitaire, 151 route de Saint Antoine Ginestière, 06202 Nice Cedex 3; Laboratoire Surfaces-Interfaces en Odontologie, Faculté de Chirurgie Dentaire, Nice, France

* Corresponding author. E-mail: fosse{at}unice.fr.

Received 13 August 2002 ; revised 9 October 2002 ; accepted 20 February 2003

Abstract

Sixty-two strains of oral (32) and non-oral (30) Prevotella producing {beta}-lactamases were screened for cfxA by PCR, using an intragenic primer pair. All 62 were cfxA/cfxA2 positive. Fourteen of these strains, representing seven pigmented and seven non-pigmented Prevotella species were submitted to further PCR with specific primers that amplified the whole {beta}-lactamase structural gene (966 bp). After cloning and sequencing, the deduced amino acid sequences were compared with that of Bacteroides vulgatus CfxA {beta}-lactamase. All 14 sequences possessed the E272K substitution characteristic of CfxA2. CfxA sensu stricto was not observed in the present series. G83D, F/V189L, W193L and D239Y substitutions were observed more than once, without species specificity. This sequence analysis indicates that most oral and non-oral {beta}-lactamase-producing Prevotella isolates from French patients produce variants of the CfxA enzyme.

Keywords: anaerobes, antibiotic resistance, {beta}-lactam, Prevotella
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