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JAC Advance Access published online on March 28, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg193
© 2003 by The British Society for Antimicrobial Chemotherapy
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© 2003 The British Society for Antimicrobial Chemotherapy

Brief report

Antimicrobial resistance in clinical isolates of Salmonella enterica serotype Enteritidis: relationships between mutations conferring quinolone resistance, integrons, plasmids and genetic types

Sara M. Soto 1, M. Angeles González-Hevia 2, M. Carmen Mendoza 1*

1 Departamento de Biología Funcional, Área de Microbiología, Facultad de Medicina, Universidad de Oviedo, C/Julián Clavería 6, 33006 Oviedo
2 Laboratorio de Salud Pública, Consejería de Sanidad, Principado de Asturias, Carretera del Rubín s/n, 33001 Oviedo, Spain

* Corresponding author. E-mail: cmendoza{at}correo.uniovi.es.

Received 2 January 2002 ; revised 25 May 2002 ; accepted 4 February 2003

Abstract

In 481 clinical isolates of Salmonella enterica serotype Enteritidis collected from a Spanish region in 2000, 108, 83 and four isolates were resistant, respectively, to nalidixic acid, ampicillin or both. Nalidixic acid resistance was the result of DNA gyrase mutations involving the codons Asp-87 (97 isolates) and Ser-83 (15 isolates) of the gyrA gene; no mutations in parC were detected. In ampicillin-resistant strains, blaTEM genes located on plasmids and/or the chromosome were implicated. Five plasmids containing blaTEM1-like genes were identified, ranging from 7 to 100 kb, four of which were self-transferable; one of these contained a class 1 sul1 integron with an aadA1a gene cassette. This integron was also found on the chromosome of an isolate resistant to ampicillin, streptomycin and sulfadiazine. A relationship between a 40 kb self-transferable plasmid and strains of Salmonella Enteritidis phage type 6a with a distinctive RAPD profile was established.


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