Skip Navigation



JAC Advance Access published online on March 13, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg173
© 2003 by The British Society for Antimicrobial Chemotherapy
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
51/4/991    most recent
dkg173v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Morita, Y.
Right arrow Articles by Tsuchiya, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morita, Y.
Right arrow Articles by Tsuchiya, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2003 The British Society for Antimicrobial Chemotherapy

Brief report

Induction of mexCD-oprJ operon for a multidrug efflux pump by disinfectants in wild-type Pseudomonas aeruginosa PAO1

Yuji Morita 1, Takeshi Murata 2, Takehiko Mima 1, Sumiko Shiota 1, Teruo Kuroda 1, Tohru Mizushima 1, Naomasa Gotoh 2, Takeshi Nishino 2, Tomofusa Tsuchiya 1*

1 Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700-8530
2 Department of Microbiology, Kyoto Pharmaceutical University, Yamashina, Kyoto 607-8414, Japan

* Corresponding author. E-mail: tsuchiya{at}pharm.okayama-u.ac.jp.

Received 11 December 2002 ; revised 24 December 2002 ; accepted 22 January 2003

Abstract

Induction of the MexCD-OprJ multidrug efflux pump was investigated in wild-type Pseudomonas aeruginosa PAO1. MexCD-OprJ was induced by clinically important disinfectants such as benzalkonium chloride and chlorhexidine gluconate, and by some cytotoxic agents such as tetraphenylphosphonium chloride, ethidium bromide and rhodamine 6G. MexCD-OprJ was not induced by norfloxacin, tetracycline, chloramphenicol, streptomycin, erythromycin or carbenicillin, although they are substrates for the pump. Cells of PAO1 showed increased resistance to norfloxacin when grown in the presence of the inducers of the mexCD-oprJ operon mentioned above. These results indicate that MexCD-OprJ plays an important role in intrinsic multidrug resistance in wild-type P. aeruginosa in hospitals where disinfectants are used frequently.

Keywords: MexCD-OprJ, multidrug efflux pump, inducers, P. aeruginosa
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
S. Fraud, A. J. Campigotto, Z. Chen, and K. Poole
MexCD-OprJ Multidrug Efflux System of Pseudomonas aeruginosa: Involvement in Chlorhexidine Resistance and Induction by Membrane-Damaging Agents Dependent upon the AlgU Stress Response Sigma Factor
Antimicrob. Agents Chemother., December 1, 2008; 52(12): 4478 - 4482.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
N. Mesaros, Y. Glupczynski, L. Avrain, N. E. Caceres, P. M. Tulkens, and F. Van Bambeke
A combined phenotypic and genotypic method for the detection of Mex efflux pumps in Pseudomonas aeruginosa
J. Antimicrob. Chemother., March 1, 2007; 59(3): 378 - 386.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
E. Marrer, K. Schad, A. T. Satoh, M. G. P. Page, M. M. Johnson, and L. J. V. Piddock
Involvement of the Putative ATP-Dependent Efflux Proteins PatA and PatB in Fluoroquinolone Resistance of a Multidrug-Resistant Mutant of Streptococcus pneumoniae
Antimicrob. Agents Chemother., February 1, 2006; 50(2): 685 - 693.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
H. Sekiya, T. Mima, Y. Morita, T. Kuroda, T. Mizushima, and T. Tsuchiya
Functional Cloning and Characterization of a Multidrug Efflux Pump, MexHI-OpmD, from a Pseudomonas aeruginosa Mutant
Antimicrob. Agents Chemother., September 1, 2003; 47(9): 2990 - 2992.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.