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JAC Advance Access published online on March 13, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg150
© 2003 by The British Society for Antimicrobial Chemotherapy
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© 2003 The British Society for Antimicrobial Chemotherapy

Brief report

Isolation, excystation and axenization of Giardia lamblia isolates: in vitro susceptibility to metronidazole and albendazole

Agostinho Cruz 1*, M. Isaura Sousa 2, Zaida Azeredo 3, Emilia Leite 4, J. C. Figueiredo de Sousa 2, Miguel Cabral 2

1 Escola Superior de Tecnologia da Saúde do Porto, Rua João Oliveira Ramos 87, 4000-294 Porto; Instituto de Ciências Biomédicas de Abel Salazar, 4099-003 Porto
2 Laboratório de Microbiologia, Faculdade de Farmácia da Universidade do Porto, 4050-047 Porto, Portugal
3 Instituto de Ciências Biomédicas de Abel Salazar, 4099-003 Porto
4 Escola Superior de Tecnologia da Saúde do Porto, Rua João Oliveira Ramos 87, 4000-294 Porto

* Corresponding author. E-mail: agostinho-cruz{at}clix.pt.

Received 11 January 2002 ; revised 15 May 2002 ; accepted 13 January 2003

Abstract

From 53 samples of human faeces containing Giardia lamblia cysts, 18 isolates were successfully excysted in vitro, and cultivated axenically in TYI-S-33 modified medium. The in vitro effects of metronidazole and albendazole on these isolates were evaluated by the trophozoite adherence inhibition method. The IC50 was between 2.4 and 11.5 µM for metronidazole and 0.027 and 0.192 µM for albendazole. These IC50 values were similar to those found for the ATCC 30888 and 30957 reference isolates. All isolates were susceptible to the antiparasitic drugs tested. These results suggest that resistance of G. lamblia to metronidazole and albendazole does not seem to be a significant problem in our population.

Keywords: Giardia, susceptibility assays, metronidazole, albendazole
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