JAC Advance Access published online on February 25, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg149
© 2003 by The British Society for Antimicrobial Chemotherapy
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Original article
1 Institute of Infectious
Diseases and Public Health, University of Ancona
* Corresponding author. E-mail: anconacmi{at}interfree.it.
Received 7 June 2002
; revised 26 June 2002
; accepted 13 January 2003
Two laboratory methods, a cell culture system and double
fluorogenic staining, were used to study the viability and infective
ability of Cryptosporidium parvum sporozoites and
oocysts after short-term exposure to four cathelicidin peptides.
The compounds, SMAP-29, BMAP-28, PG-1 and Bac7(1-35), exerted a
strong cytotoxic effect on sporozoites, but did not affect the viability and
function of oocysts consistently. Overall, in the sporozoite series,
a percentage of the viable population decreased rapidly to less
than detectable levels after 15 and 60 min exposure to the peptides
at concentrations of 100 and 10 µg/mL,
respectively. In the oocyst series, no compound produced complete
inhibition of parasite growth: 60-85% of the oocyst
population was viable after 180 min exposure at 100 µg/mL.
SMAP-29 exerted the highest activity against both sporozoites and
oocysts.
Keywords: cathelicidins, Cryptosporidium parvum,
susceptibility
In vitro effect on Cryptosporidium
parvum of short-term exposure to cathelicidin peptides
2 Department of Biomedical Sciences
and Technology, University of Udine
3 Department of Biomedical Sciences
and Technology, University of Udine; National Laboratory CIB, Area Science Park, Padriciano
99, 34012 Trieste, Italy
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