JAC Advance Access published online on January 28, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg097
© 2003 by The British Society for Antimicrobial Chemotherapy
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Original article
1 Department of Microbiology,
University of Seville, Apdo. 914, 41009 Seville
* Corresponding author. E-mail: felipefc{at}supercable.es.
Received 14 July 2002
; revised 25 September 2002
; accepted 20 November 2002
Twenty blood isolates of Acinetobacter
baumannii were studied, representing eight pulsed-field gel
electrophoresis patterns and all different antimicrobial susceptibility
patterns observed during 1995-97 at the University Hospital
Virgen Macarena, Seville, Spain. The MIC90s (mg/L) of imipenem
and meropenem decreased from 16 to 0.5 and from 8 to 4, respectively,
in the presence of BRL 42715 (BRL) but not clavulanic acid. Hydrolysing
activity (nmol/min/mg) of bacterial supernatants against cefaloridine
ranged from 8.8 to 552.3 for A. baumannii type
I (imipenem MICs
Keywords: Acinetobacter baumannii, carbapenems, Relationship between
-lactamase
production, outer membrane protein and penicillin-binding protein
profiles on the activity of carbapenems against clinical isolates
of Acinetobacter baumannii
2 Department of Microbiology,
University of Seville, Apdo. 914, 41009 Seville; University Hospital Virgen Macarena,
Seville
3 Center of Molecular
Biology ‘Severo Ochoa', Autonomous University
of Madrid, Madrid, Spain
2), which expressed
only a 
-lactamase of pI 
9,
and from 12.3 to 1543.5 for A. baumannii type II
(imipenem MICs 4), which expressed
a -lactamase of pI 9
and two others of pI 6.3 and 7. The hydrolysing activities of A. baumannii type II against imipenem, meropenem
and oxacillin were higher than those observed for A.
baumannii type I. Ten outer membrane protein (OMP) profiles
(A. baumannii types I and II) were visualized on
10% SDS-PAGE gels with 6 M urea, whereas only
five OMP profiles (A. baumannii types I and II)
were differentiated in 12% SDS-PAGE gels. Five A. baumannii with OMP profile type B, characterized
by the absence of a 22.5 kDa OMP, were resistant to meropenem and/or
imipenem. Twelve penicillin-binding protein (PBP) patterns were observed.
PBP patterns of A. baumannii type II were characterized
by the absence of a 73.2 kDa band (PBP 2). We concluded that production
of -lactamases of pI 6.3 and 7.0 and
reduced expression of PBP 2 are the most frequently observed mechanisms
of resistance to carbapenems. In some isolates, loss of a 22.5 kDa
OMP is also related to resistance to carbapenems.-lactamases, outer membrane proteins,
penicillin-binding proteins
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