JAC Advance Access published online on January 14, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg088
© 2003 by The British Society for Antimicrobial Chemotherapy
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Original article
1 Laboratoire de Parasitologie et Mycologie Médicales,
Centre Hospitalier Universitaire La Milétrie, BP 577,
86021 Poitiers Cedex, France
* Corresponding author. E-mail: Christine.Imbert{at}univ-poitiers.fr.
Received 2 May 2002
; revised 11 September 2002
; accepted 19 November 2002
This study investigates the influence of treatment
of plastic and extracellular matrix (ECM) proteins with chlorhexidine
or benzalkonium chloride on subsequent adherence of Candida
albicans. Three concentrations were tested for each antiseptic:
(i) chlorhexidine, MIC (6.25-12.5 mg/L), 80 x MIC
and 800 x MIC; and (ii) benzalkonium
chloride, MIC (3.12 mg/L), 40 x MIC
and 1600 x MIC. Chlorhexidine and benzalkonium
chloride activities were correlated with the tested concentrations.
Antiseptics used at MIC were unable to modify the adherence to plastic
or ECM proteins. Chlorhexidine (80 x MIC)
induced a decrease in plastic adherence of 31% of the 15
strains used and an increase in ECM protein adherence of 13% of
strains. Benzalkonium chloride (40 x MIC) induced
a decrease in adherence to ECM proteins or plastic of 13-27% of
strains. Our results indicated that the treatment with 1600 x MIC benzalkonium chloride could induce
the opposite effect on adherence, depending on the surface: 60% of
the strains showed an increase in their adherence to ECM proteins,
whereas 93% of the strains showed a decrease in their adherence
to plastic. A similar phenomenon was observed after treatment
with 800 x MIC chlorhexidine: 60% of
the strains showed an increase in their adherence to ECM proteins,
whereas 67% showed a decrease in adherence to plastic.
Treatment of medical devices with at least 5000 mg/L of chlorhexidine
or benzalkonium chloride could therefore reduce C. albicans adherence
to plastic surfaces, but would be unable to prevent fungal adherence
to ECM proteins.
Keywords: Candida, adherence, antiseptic
agents
Treatment of plastic and extracellular matrix components
with chlorhexidine or benzalkonium chloride: effect on Candida
albicans adherence capacity in vitro
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Zumbuehl, L. Ferreira, D. Kuhn, A. Astashkina, L. Long, Y. Yeo, T. Iaconis, M. Ghannoum, G. R. Fink, R. Langer, et al. Antifungal hydrogels PNAS, August 7, 2007; 104(32): 12994 - 12998. [Abstract] [Full Text] [PDF]
|
|