JAC Advance Access published online on January 6, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg076
© 2003 by The British Society for Antimicrobial Chemotherapy
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Original article
1 Clinical Microbiology and Public Health Laboratory, Addenbrooke's
Hospital, Cambridge CB2 2QW, UK
* Corresponding author. E-mail: dfjb2{at}cam.ac.uk.
Received 11 April 2002
; revised 27 August 2002
; accepted 4 November 2002
Objectives: Vancomycin-resistant enterococcus
enrichment broth (VEB) and vancomycin-resistant enterococcus selective
agar with vancomycin 6 mg/L (VSA) are novel azide-aesculin agar-based
media that contain meropenem as an additional selective agent. The
media were compared with enterococcosel broth (EB) and enterococcosel
agar with vancomycin 6 mg/L (EA) for the isolation of glycopeptide-resistant
enterococci (GRE) from routine faecal screening specimens. Methods: Two hundred and eighteen routine faecal
screening specimens from patients at Addenbrooke's Hospital
were examined. The majority were from patients on haematology wards (155)
or the intensive therapy unit (ITU) (21). Specimens were inoculated
on to VSA and EA directly, and after enrichment in VEB and EB, respectively. Results: One hundred and twenty-eight GRE isolates
were recovered from 93 (43%) specimens with enterococci
carrying vanA or vanB genes. There
were no statistically significant differences between media (specimens
positive; numbers of GRE isolates) on direct plating on VSA (87;
104) or EA (86; 97) or following 24 h enrichment in VEB (89; 103)
or EB (86; 98). There was no significant advantage to enrichment
compared with direct plating. Incubation of enrichment broth cultures
for only 6 h appeared detrimental. Enterococci with vanC were
isolated significantly less frequently from VEB and VSA than from
EB and EA. Growth of organisms other than GRE was more common on
VSA than on EA. Conclusions: VEB and VSA were at least as effective
as EB and EA for the recovery of GRE from faecal screening
specimens, but substantially more non-GRE grew on VSA than on EA. Enrichment
culture offered no significant advantages over direct plating.
Evaluation of selective and enrichment media for
isolation of glycopeptide-resistant enterococci from faecal specimens
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