JAC Advance Access published online on January 6, 2003
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg057
© 2003 by The British Society for Antimicrobial Chemotherapy
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Original article
1 Institute of Infections
and Immunity, Floor C, West Block, University Hospital, Queen's
Medical Centre,
University of Nottingham
* Corresponding author. E-mail: Peter.Jenks{at}nottingham.ac.uk.
Received 3 September 2002
; revised 10 October 2002
; accepted 23 October 2002
Objective: To determine the ability
of mastic monotherapy to eradicate Helicobacter pylori infection
from mice. Materials and methods: The susceptibility of H. pylori SS1 to mastic was assessed by broth dilution
determination of the MIC and MBC. Mice were inoculated intragastrically
with either a suspension of H. pylori SS1 (n = 70)
or brain-heart infusion broth alone (n = 10).
Mice were given antimicrobial chemotherapy 4 weeks after infection
and were administered the mouse equivalent of either 2 g of mastic
twice daily for 7 days or a triple therapy regimen containing the
mouse equivalent of 400 mg of metronidazole, 250 mg of clarithromycin
and 20 mg of omeprazole twice daily for 7 days. Mice were killed
either immediately or 1 month after the completion of treatment, and
their stomachs cultured for H. pylori. Results: The mastic MIC and MBC of H.
pylori SS1 were 7.80 and 31.25 mg/L, respectively.
The triple therapy regimen eradicated infection from 19 of 20
SS1-infected mice. Mastic failed to eradicate infection from any
of the 18 SS1-infected mice (P < 0.001)
and there was no signifi- cant reduction in gastric bacterial
load in mice treated with this regimen. Conclusion: Despite reported beneficial effects
in ulcer patients and the good in vitro activity
of mastic against H. pylori, this compound is unable
to eradicate H. pylori infection from mice.
Keywords: mastic, Helicobacter pylori,
mouse model, eradication
Monotherapy with mastic does not eradicate Helicobacter
pylori
infection from mice
2 Division
of Microbiology and Infectious Diseases, University of Nottingham,
Nottingham NG7 2UH, UK
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Paraschos, P. Magiatis, S. Mitakou, K. Petraki, A. Kalliaropoulos, P. Maragkoudakis, A. Mentis, D. Sgouras, and A.-L. Skaltsounis In Vitro and In Vivo Activities of Chios Mastic Gum Extracts and Constituents against Helicobacter pylori Antimicrob. Agents Chemother., February 1, 2007; 51(2): 551 - 559. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Bebb, N. Bailey-Flitter, D. Ala'Aldeen, and J. C. Atherton Mastic gum has no effect on Helicobacter pylori load in vivo J. Antimicrob. Chemother., September 1, 2003; 52(3): 522 - 523. [Abstract] [Full Text] [PDF]
|
|