JAC Advance Access published online on December 12, 2002
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg022
© 2002 by The British Society for Antimicrobial Chemotherapy
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Original Paper
1 Anaerobe Reference Laboratory,
Department of Microbiology, National Public Health Institute, Mannerheimintie 166,
FIN-00300 Helsinki, Finland
* Corresponding author. E-mail: SusanNyforsUK{at}aol.com.
Received 13 May 2002
; revised 2 September 2002
; accepted 26 September 2002
Penicillin resistance due to
Keywords: Fusobacterium nucleatum, in vitro susceptibility, MIC, penicillin resistance, Emergence of penicillin resistance among Fusobacterium
nucleatum populations of commensal oral flora during early
childhood
2 Anaerobe Reference Laboratory,
Department of Microbiology, National Public Health Institute, Mannerheimintie 166,
FIN-00300 Helsinki, Finland; Department of Surgical Sciences, Kuwait University,
PO Box 24923, Safat 13110, Kuwait
3 Department
of Vaccines, National Public Health Institute, Mannerheimintie 166,
FIN-00300 Helsinki, Finland
4 Department
of Education, University of Helsinki,
PO Box 39, FIN-00014 Helsinki, Finland
-lactamase
production is surprisingly common among oral bacteria in childhood. Fusobacterium nucleatum, a Gram-negative anaerobic
bacillus, is a member of the developing oral commensal flora. As
part of the investigation on the emergence of oral bacterial resistance,
the aim of the present study was to examine longitudinally the penicillin
resistance among salivary F. nucleatum populations
as related to age, day care attendance and sibling history, and
exposure to antimicrobial agents. Altogether 1492 F.
nucleatum isolates from saliva of 44 healthy infants followed
at a study clinic at 2, 6, 12, 18 and 24 months of age were tested
for -lactamase production. Furthermore,
the 276 -lactamase-positive isolates
were examined for their in vitro susceptibility
to penicillin G by the NCCLS-approved agar dilution method. Statistical
analysis of the associations between penicillin-resistant isolates
and infants' age, day care attendance, number of siblings
and their ear infections, and exposure to antimicrobial agents was
performed by SPSS Windows Version 10. The prevalence of infants
harbouring -lactamase-producing F. nucleatum strains increased from 2% to
49% during the follow-up time. In nearly all cases -lactamase-producing F.
nucleatum isolates were found simultaneously with -lactamase-negative
isolates. Most -lactamase-producing
isolates (80%) showed an MIC of 
8
mg/L. In conclusion, the prevalence of infants harbouring penicillin-resistant
F. nucleatum due to -lactamase
production increased with age and usage of antimicrobial agents
during the first year of life.-lactamase production
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