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JAC Advance Access published online on November 28, 2002
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg016
© 2002 by The British Society for Antimicrobial Chemotherapy
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© 2002 The British Society for Antimicrobial Chemotherapy

In Brief

Comparison of levels of HIV-1 resistance to protease inhibitors by recombinant versus conventional virus phenotypic assay and two genotypic interpretation procedures in treatment-naive and HAART-experienced HIV-infected patients

Stefania Paolucci 1, Fausto Baldanti 2, Maurizio Zavattoni 3, Giuditta Comolli 3, Nazzarena Labò 3, Stefano Menzo 4, Massimo Clementi 5, Giuseppe Gerna 3*

1 Servizio di Virologia, IRCCS Policlinico San Matteo, 27100 Pavia
2 Servizio di Virologia and Laboratori Sperimentali di Ricerca, IRCCS Policlinico San Matteo, 27100 Pavia
3 Servizio di Virologia, IRCCS Policlinico San Matteo, 27100 Pavia
4 Istituto di Microbiologia, Università di Ancona, Ancona
5 Università Vita-Salute San Raffaele, IRCCS San Raffaele, Milan, Italy

* Corresponding author. E-mail: g.gerna{at}smatteo.pv.it.

Received 15 March 2002 ; revised 22 July 2002 ; accepted 27 September 2002

Abstract

Objectives: To compare genotypic and phenotypic HIV-1 drug resistance assays.

Methods: Protease inhibitor (PI) susceptibility was phenotypically analysed in HIV-1 isolates and recombinant viruses expressing proteases from viral isolates. Two genotypic interpretation methods were carried out in parallel.

Results: Entirely concordant resistance levels were shown in 5/10 (50%) highly active anti-retroviral therapy (HAART)-experienced patients, whereas minor discrepancies were observed in the remaining five patients.

Conclusions: The four assays provide comparable results. The recombinant virus phenotypic assay may provide the most accurate evaluation of resistance; however, genotypic interpretation procedures are helpful for daily therapeutic decisions.


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