Skip Navigation



JAC Advance Access published online on November 28, 2002
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkg015
© 2002 by The British Society for Antimicrobial Chemotherapy
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
51/1/39    most recent
dkg015v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Tristram, S. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tristram, S. G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2002 The British Society for Antimicrobial Chemotherapy

Original Paper

Effect of extended-spectrum {beta}-lactamases on the susceptibility of Haemophilus influenzae to cephalosporins

Stephen G. Tristram 1*

1 School of Human Life Sciences, University of Tasmania, Launceston, Tasmania 7250, Australia

* Corresponding author. E-mail: Stephen.Tristram{at}utas.edu.au.

Received 10 July 2002 ; revised 4 September 2002 ; accepted 25 September 2002

Abstract

The extended-spectrum {beta}-lactamases (ESBLs) TEM-3, TEM-4 and TEM-5 were cloned into Haemophilus influenzae. These recombinant strains exhibited cefotaxime MICs of 0.5, 0.25 and 0.12 mg/L for TEM-3, -4 and -5, respectively, and the MIC of cefaclor was 4.0 mg/L. These MICs are higher than those of {beta}-lactamase-negative strains, or those producing simple wild-type TEM-1 {beta}-lactamase, but not high enough to be categorized as resistant according to the breakpoints of the NCCLS. The clones were also categorized as susceptible using NCCLS disc diffusion methodology and interpretive criteria. This study shows that current NCCLS susceptibility testing methods may have difficulty in detecting ESBLs if they were to occur in H. influenzae.

Keywords: extended-spectrum {beta}-lactamase, TEM {beta}-lactamase
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
S. G. Tristram, M. J. Pitout, K. Forward, S. Campbell, S. Nichols, and R. J. Davidson
Characterization of extended-spectrum {beta}-lactamase-producing isolates of Haemophilus parainfluenzae
J. Antimicrob. Chemother., March 1, 2008; 61(3): 509 - 514.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
S. G. Tristram and J. G. Burdach
Effect of cloned inhibitor-resistant TEM {beta}-lactamases on the susceptibility of Haemophilus influenzae to amoxicillin/clavulanate
J. Antimicrob. Chemother., November 1, 2007; 60(5): 1151 - 1154.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
S. Tristram, M. R. Jacobs, and P. C. Appelbaum
Antimicrobial Resistance in Haemophilus influenzae
Clin. Microbiol. Rev., April 1, 2007; 20(2): 368 - 389.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
B. Bozdogan, S. Tristram, and P. C. Appelbaum
Combination of altered PBPs and expression of cloned extended-spectrum {beta}-lactamases confers cefotaxime resistance in Haemophilus influenzae
J. Antimicrob. Chemother., April 1, 2006; 57(4): 747 - 749.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
S. G. Tristram, R. Hawes, and J. Souprounov
Variation in selected regions of blaTEM genes and promoters in Haemophilus influenzae
J. Antimicrob. Chemother., September 1, 2005; 56(3): 481 - 484.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
S. G. Tristram, B. Bozdogan, and P. C. Appelbaum
Disc diffusion-based screening tests for extended-spectrum {beta}-lactamases in Haemophilus influenzae
J. Antimicrob. Chemother., April 1, 2005; 55(4): 570 - 573.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J.-C. Galan, M.-I. Morosini, M.-R. Baquero, M. Reig, and F. Baquero
Haemophilus influenzae blaROB-1 Mutations in Hypermutagenic {Delta}ampC Escherichia coli Conferring Resistance to Cefotaxime and {beta}-Lactamase Inhibitors and Increased Susceptibility to Cefaclor
Antimicrob. Agents Chemother., August 1, 2003; 47(8): 2551 - 2557.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.