JAC Advance Access published online on November 18, 2002
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkf222
© 2002 by The British Society for Antimicrobial Chemotherapy
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Original Paper
1 Kumamoto Prefectural Fisheries
Research Center, Ohyano, Amakusa, Kumamoto 869-3603, Japan; Laboratory of Marine Resource Chemistry,
Graduate School of Agriculture, Kyushu University, 6-10-1, Hakozaki,
Fukuoka 812-8581, Japan
* Corresponding author. E-mail: nagayama{at}jade.dti.ne.jp.
Received 2 January 2002
; revised 10 August 2002
; accepted 28 August 2002
The bactericidal activity of phlorotannins from brown
algae against food-borne pathogenic bacteria (25 strains), methicillin-resistant Staphylococcus aureus (MRSA) (nine strains) and Streptococcus pyogenes (one strain) was examined
and compared with that of catechins. In addition, the effect of
the oral administration of phlorotannins on mice was investigated.
Phlorotannins, which are oligomers of phloroglucinol, were extracted
from thalli of the brown alga Ecklonia kurome and
prepared by silicic acid chromatography. The bactericidal activity
of polyphenols was determined using a broth microdilution method.
Of the bacteria tested, Campylobacter spp. were
the most susceptible to the phlorotannins. The MBCs of the crude phlorotannins,
dieckol and 8,8'-bieckol (hexamers),
and that of epigallocatechin gallate (EGCG) against Campylobacter
jejuni were 50 mg/L, 0.03 µmol/mL
and 0.03 µmol/mL, respectively. On the
whole, the bactericidal effects of the phlorotannins were more pronounced
than those of the catechins. The phlorotannins were as effective
against MRSA as against the other bacteria tested. At twice the
MBCs, all Vibrio parahaemolyticus were killed within
0.5-2 h. However, at the same concentration, catechins
showed little bactericidal activity within 4 h. No effect on mice was
observed with oral administration of the phlorotannins under the
conditions tested.
Bactericidal activity of phlorotannins from the
brown alga
Ecklonia kurome
2 The Chemo-Sero-Therapeutic Research
Institute, Kikuchi Research Center, Kyokushi, Kikuchi,
Kumamoto 869-1298, Japan
3 Laboratory of Marine Resource Chemistry,
Graduate School of Agriculture, Kyushu University, 6-10-1, Hakozaki,
Fukuoka 812-8581, Japan
4 Kumamoto Prefectural Fisheries
Research Center, Ohyano, Amakusa, Kumamoto 869-3603, Japan
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