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JAC Advance Access published online on October 22, 2002
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkf208
© 2002 by The British Society for Antimicrobial Chemotherapy
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© 2002 The British Society for Antimicrobial Chemotherapy

In Brief

Characterization of a laboratory-generated variant of BPS ß-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime

P. L. Ho 1*, Terence K. M. Cheung 1, W. C. Yam 1, K. Y. Yuen 2

1 Division of Infectious Diseases, Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Pokfulam, Hong Kong SAR, China
2 HKU-Pasteur Research Centre, Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam Road, Pokfulam, Hong Kong SAR, China

* Corresponding author. E-mail: plho{at}hkucc.hku.hk.

Received 11 April 2002 ; revised 20 August 2002

Abstract

Burkholderia pseudomallei produces an Ambler class A ß-lactamase, known as BPS-1. The ß-lactamase gene from a laboratory-derived, ceftazidime-resistant strain of B. pseudomallei (LH-1-2) was cloned and expressed in Escherichia coli. The ß-lactamase, named BPS-1m, had an identical isoelectric focusing point (pI 7.7) to that of BPS-1, but differed in having a stronger hydrolytic activity against ceftazidime. Susceptibility testing showed that BPS-1m when expressed in E. coli conferred resistance to ceftazidime (MIC >= 32 mg/L). The amino acid sequence of BPS-1m differed from that of BPS-1 by a Pro-to-Ser change at position 167 in the omega loop.


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