JAC Advance Access published online on September 20, 2002
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkf187
© 2002 by The British Society for Antimicrobial Chemotherapy
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In Brief
1 Servicio de Micología, Centro Nacional de Microbiología,
Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, 28220
Majadahonda, Madrid, Spain
* Corresponding author. E-mail: juanl.rodriguez-tudela{at}isciii.es.
Received 5 April 2002
; revised 18 June 2002
; accepted 26 July 2002
Two different methods of inoculum preparation for susceptibility
testing were analysed. The first method was adjustment of inoculum
size by haemocytometer counting. The second method was spectrophotometric
adjustment at 530 nm. The reliability of both methods was assessed
by colony counting. The overall agreement between colony counting
and haemocytometer counts was 93.6%, and the intraclass
coefficient correlation was 0.71 (P < 0.05).
Pearson's correlation index between colony counts and optical
density values was -0.059 (P > 0.05).
Optical densities ranged between 0.01 and 1.2, showing less reproducibility
than expected by the NCCLS M38-P standard. Haemocytometer counting
is a more reliable method of inoculum preparation for antifungal
susceptibility testing.
Comparative evaluation of two different methods
of inoculum preparation for antifungal susceptibility testing of
filamentous fungi
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