JAC Advance Access published online on September 20, 2002
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkf181
© 2002 by The British Society for Antimicrobial Chemotherapy
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Original Paper
1 Department of Microbiology,
City Hospital NHS Trust, Birmingham, UK
* Corresponding author. E-mail: jenny.andrews{at}cityhospbham.wmids.nhs.uk.
Received 25 February 2002
; revised 15 May 2002
; accepted 18 July 2002
BSAC methodology was used to study media available
from four manufacturers for susceptibility testing of Pseudomonas
aeruginosa in the UK and Ireland. Fifty isolates of P.
aeruginosa, including ATCC and NCTC control strains, were studied
by disc testing methods by 20 centres around the UK and Ireland
and by MIC determination at the BSAC Standardized Method Development
Centre (SMDC) in Birmingham, UK. Although many of the antibiotics
tested gave similar zone diameters for a particular strain-antibiotic
combination irrespective of the medium used for testing, significant
differences in media performance were noted for the aminoglycosides, imipenem
and colistin. The data generated showed that for testing aminoglycosides,
zone diameters on two of the agars were significantly larger than
those on Oxoid IsoSensitest agar—the recommended medium
for the BSAC method. For imipenem, zones were smaller on one of
the agars and for colistin, zones were larger on one of the agars
in comparison with those on Oxoid agar. Scattergram analysis of
zone diameters and MICs of colistin indicated that the zone diameter
breakpoints should be adjusted. Media purported to be equivalent
to Oxoid IsoSensitest agar do not give the same zone sizes with
some agents and should not be used until standards for media performance
have been established and the media have been shown to perform adequately.
Evaluation of media available for testing the susceptibility
of Pseudomonas aeruginosa by BSAC
methodology
2 Department of Microbiology,
St Thomas' Hospital, London, UK
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