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JAC Advance Access originally published online on May 27, 2009
Journal of Antimicrobial Chemotherapy 2009 64(2):274-277; doi:10.1093/jac/dkp194
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© The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains

Olivier Clermont1, Hiran Dhanji2, Mathew Upton3, Tarek Gibreel3, Andrew Fox4, David Boyd5, Michael R. Mulvey5, Patrice Nordmann6, Etienne Ruppé7, Jean Louis Sarthou7, Thierry Frank8,9, Sophie Vimont8,10, Guillaume Arlet8,10, Catherine Branger1,11, Neil Woodford2 and Erick Denamur1,*

1 INSERM U722 and Université Paris 7, Faculté de Médecine, Site Xavier Bichat, 75018 Paris, France 2 Antibiotic Resistance Monitoring and Reference Laboratory, Centre for Infections, Health Protection Agency, London NW9 5HT, UK 3 Department of Medical Microbiology, School of Medicine, University of Manchester, Manchester M13 9WL, UK 4 Health Protection Agency, North West Laboratory, Manchester M13 9WZ, UK 5 Antimicrobial Resistance and Nosocomial Infections, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada 6 Département de Bactériologie–Virologie, INSERM U914, Université Paris Sud, Hôpital de Bicêtre, Le-Kremlin-Bicêtre, France 7 Institut Pasteur du Cambodge, Phnom Penh, Cambodia 8 Université Paris 6, Faculté de Médecine, Département de Microbiologie, UPRES EA2392, Paris, France 9 Institut Pasteur de Bangui, Bangui, Central African Republic 10 AP-HP, Hôpital Tenon, Service de Bactériologie, Paris, France 11 AP-HP, Hôpital Louis Mourier, Service de Microbiologie–Hygiène, Colombes, France

Received 1 April 2009; returned 15 April 2009; revised 28 April 2009; accepted 30 April 2009

* Corresponding author. Tel: +33-1-57-27-77-39; Fax: +33-1-57-27-75-21; E-mail: erick.denamur{at}inserm.fr

Objectives: Recently, a CTX-M-15 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with a high virulence potential, has been reported all over the world, representing a major public health problem. The present study was carried out to develop a rapid and simple detection assay that identifies members of this clone.

Methods: A total of 627 E. coli isolates of which 373 produced an ESBL, collected across four continents, were screened using a O25b-ST131 clone allele-specific PCR for the pabB gene.

Results: One hundred and forty-three ESBL isolates were found positive with the assay. These isolates were all of O25b type and, when studied by multilocus sequence typing (25 cases), were all of ST131. The O25b-ST131 clone was found to produce ESBLs other than CTX-M-15, specifically CTX-M-2, -3, -14, -27, -32 and -61 as well as TEM-24. This clone represents 3% of non-ESBL B2 isolates originating from urinary tract infections in Paris.

Conclusions: We have developed a PCR-based assay that easily identifies a clone with high likelihood of producing ESBLs, including CTX-M-15.

Keywords: B2 phylogenetic subgroup I , ESBL , O25 , ST 131


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